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• W2320567634 abstract "Event Abstract Back to Event Integration of multimodal neuroanatomical data of gray short-tailed opossum Piotr Majka1*, Natalia Chlodzinska1, Tomasz Banasik2, Ruzanna L. Djavadian1, Władysław P Węglarz2, Krzysztof Turlejski1 and Daniel K. Wójcik1 1 Nencki Institute of Experimental Biology, Poland 2 H. Niewodniczanski Institute of Nuclear Physics PAN, Poland The gray short-tailed opossum (Monodelphis domestica) becomes increasingly popular laboratory animal. This species is particularly useful in developmental studies because of the precocious stage of development of newborns. Although a large number of neuroanatomical studies were performed on this species, a consistent and comprehensive digital neuroanatomical reference is not available. Digital brain atlases are very different from their traditional book predecessors. Conventional 2D atlases are based on collections of delineated microphotographs of series of stained brain slices. Digital three-dimensional brain atlases allow unconstrained navigation through brain volume and reslicing at arbitrary angles. Furthermore, combining data from different specimens or modalities, localizing and analyzing data within the context of brain volume e.g. performing morphometric analysis or assessing intergroup variances quantitatively, is far more natural in the 3D context. The aim of this study is to integrate multimodal neuroanatomical data of gray short-tailed opossum. The data include: (1) MRI images collected using Bruker BioSpec 9.4T imaging system: T2 weighted, 100um isotropic in vivo and T1/T2* weighted, 50um isotropic taken after 48 hours and 30 days post mortem. (2) Photographs of the tissue block taken during cryosectioning of the brains ('blockface' images). (3) Microphotographs of slices stained with histological methods including Nissl and Acetylocholinesterase. The collected data were processed to merge different modalities. In this process the blockface volume, as an intermediate modality between MRI and histology, was the key element. In the workflow, the first step is to reconstruct and segment blockface images. Then, to eliminate global deformations related to brain fixation and its extraction from skull, in vivo and ex vivo MRI images were brought into blockface volume. Afterwards, images of stained sections and corresponding blockface images were nonlinearly registered eliminating slice-specific deformations due to staining procedures. These steps fulfilled the task of converging multimodal data into a single volumetric template. The integrated data will be used for identification and delineation of anatomical structures eventually forming a digital atlas. The project is partly supported by an infrastructural grant from the Polish Ministry of Regional Development POIG.02.03.00-00-003/09. Keywords: digital atlasing, neuroanatomical data mining, gray short-tailed opossum, 3D atlasing, Neuroimaging Conference: 5th INCF Congress of Neuroinformatics, Munich, Germany, 10 Sep - 12 Sep, 2012. Presentation Type: Poster Topic: Neuroinformatics Citation: Majka P, Chlodzinska N, Banasik T, Djavadian R, Węglarz W, Turlejski K and Wójcik D (2014). Integration of multimodal neuroanatomical data of gray short-tailed opossum. Front. Neuroinform. Conference Abstract: 5th INCF Congress of Neuroinformatics. doi: 10.3389/conf.fninf.2014.08.00081 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 21 Mar 2013; Published Online: 27 Feb 2014. * Correspondence: Dr. Piotr Majka, Nencki Institute of Experimental Biology, Warsaw, Poland, p.majka@nencki.edu.pl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Piotr Majka Natalia Chlodzinska Tomasz Banasik Ruzanna L. Djavadian Władysław P Węglarz Krzysztof Turlejski Daniel K. Wójcik Google Piotr Majka Natalia Chlodzinska Tomasz Banasik Ruzanna L. Djavadian Władysław P Węglarz Krzysztof Turlejski Daniel K. Wójcik Google Scholar Piotr Majka Natalia Chlodzinska Tomasz Banasik Ruzanna L. Djavadian Władysław P Węglarz Krzysztof Turlejski Daniel K. Wójcik PubMed Piotr Majka Natalia Chlodzinska Tomasz Banasik Ruzanna L. Djavadian Władysław P Węglarz Krzysztof Turlejski Daniel K. Wójcik Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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• W2320567634 title "Integration of multimodal neuroanatomical data of gray short-tailed opossum" @default.
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