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• W2320909364 abstract "Objectives: Respiratory gating with and without controlled ventilation has been applied for in vivo micro-computed tomography (micro-CT) of thoracic and abdominal structures in mice. We describe a simplified method for intubation and demonstrate its applicability for single-breath-hold micro-CT in mice. Methods: Mice (n = 10) were anesthetized, intubated, ventilated, and relaxed by intraperitoneal administration of rocuronium. Contrast-enhanced micro-CT of the complete thorax including the upper abdominal organs (80 kV; 37.5 μA; 190-degree rotation; 600 projections/20 seconds or 1200 projections/40 seconds; 39 × 39 × 50-μm voxel size) was performed with and without single-breath-hold technique. Results: The simplified method of intubation was fast (<1 minute) and required no special hardware in all mice. Relaxation of mice allowed prolonged single-breath-hold imaging of up to 40 seconds. Diameter of smallest identifiable lung vessels was 100 μm. Conclusions: The presented simplified method for intubation in mice is fast, safe, and effective. Additional relaxation allowed high-resolution single-breath-hold micro-CT in mice." @default.
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• W2320909364 date "2010-09-01" @default.
• W2320909364 modified "2023-10-16" @default.
• W2320909364 title "High-Speed Single-Breath-Hold Micro-Computed Tomography of Thoracic and Abdominal Structures in Mice Using a Simplified Method for Intubation" @default.
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• W2320909364 doi "https://doi.org/10.1097/rct.0b013e3181e1050a" @default.
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