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- W2321045118 abstract "We have previously shown that a biotin ligand tethered to the rim of an OmpG nanopore can be used to detect biotin-binding proteins. Here, we investigate the effect of the length of the polyethylene glycol tether on the nanopore's sensitivity and selectivity. When the tether length was increased from 2 to 45 ethylene repeats, sensitivity decreased substantially for a neutral protein streptavidin and slightly for a positively charged protein (avidin). In addition, we found that two distinct avidin binding conformations were possible when using a long tether. These conformations were sensitive to the salt concentration and applied voltage. Finally, a longer tether resulted in reduced sensitivity due to slower association for a monoclonal anti-biotin antibody. Our results highlight the importance of electrostatic, electroosmotic and electrophoretic forces on nanopore binding kinetics and sensor readout." @default.
- W2321045118 created "2016-06-24" @default.
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- W2321045118 date "2016-04-13" @default.
- W2321045118 modified "2023-10-01" @default.
- W2321045118 title "Tuning the Selectivity and Sensitivity of an OmpG Nanopore Sensor by Adjusting Ligand Tether Length" @default.
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- W2321045118 doi "https://doi.org/10.1021/acssensors.6b00014" @default.
- W2321045118 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4972499" @default.
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