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- W2321167388 abstract "Burkitt's lymphoma Raji cells were mutanized with ethylmethanesulfonate (EMS) and selected in the presence of 6-thioguanine (6-TG). The most rapidly growing 6-TG-resistant clone (NIR10-2 cells) was obtained. The characteristics of the NIR10-2 cells were as follows:1 The NIR10-2 cells were deficient in the hypoxanthine-guanine phosphoribosyl transferase (HGPRT) activity, because this cell line was sensitive to HAT selective medium.2 Enzyme-linked immunosorbent assay (ELISA) found that the NIR10-2 cells did not produce human Ig in the culture medium.3 The NIR10-2 cells grew well in the new culture medium, GIT.These results suggest that the NIR10-2 cells are suitable for human-human hybridoma production." @default.
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- W2321167388 date "1988-01-01" @default.
- W2321167388 modified "2023-09-25" @default.
- W2321167388 title "Establishment of a HAT selective medium-sensitive mutant human lymphoma cell line." @default.
- W2321167388 doi "https://doi.org/10.3412/jsb.43.577" @default.
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