Matches in SemOpenAlex for { <https://semopenalex.org/work/W2321167647> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2321167647 abstract "Capsaicin, the activator of the noxious heat sensor TPRV1 is clinically used to relieve chronic pain. Desensitization of TRPV1 involves downstream PLCδ activation, but the mechanism how capsaicin treatment can also alleviate mechanical allodynia is unknown. Piezo2 encodes rapidly adapting mechanically activated currents in sensory neurons. Expression of these novel mechanosensitive ion channels in a subset of TPRV1 positive neurons suggests their role in pain related sensory mechanotransduction.Our study shows that capsaicin application inhibited rapidly adapting mechanically activated currents in TRPV1 expressing sensory neurons. We also show that TRPV1 activation inhibited heterologously expressed Piezo1 and Piezo2 ion channels in whole-cell patch clamp experiments. Inclusion of either phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] or its precursor PI(4)P in the patch pipette alleviated this inhibitory effect. Activation of PLCβ by stimulating muscarinic receptors only marginally inhibited mechanically activated Piezo1 currents. Experiments using phosphoinositide sensors revealed that activation of PLCδ by a robust calcium influx through TRPV1 severely depleted PI(4,5)P2 and PI(4)P. On the other hand, muscarinic stimulation of PLCβ significantly decreased PI(4,5)P2 levels, but only induced a small decrease of PI(4)P. This differential activation of PLC isoforms may explain the difference between the inhibitory effects of these PLC pathways. Targeted depletion of PI(4,5)P2 and PI(4)P using a chemically inducible lipid phosphatase replicated the inhibition of Piezo1 currents. Additionally, PI(4,5)P2 and PI(4)P applied to excised inside-out patches inhibited the rundown of Piezo1 activity further emphasizing the significance of these phosphoinositides in Piezo channel regulation.Here we demonstrate that the activity of Piezo channels require the presence of either PI(4,5)P2 or PI(4)P and severe depletion of both phosphoinositides by TRPV1 activation limits channel activity. In conclusion our data may explain how capsaicin alleviates mechanically induced pain." @default.
- W2321167647 created "2016-06-24" @default.
- W2321167647 creator A5003816641 @default.
- W2321167647 creator A5014441183 @default.
- W2321167647 creator A5068881900 @default.
- W2321167647 date "2015-01-01" @default.
- W2321167647 modified "2023-09-28" @default.
- W2321167647 title "Calcium Influx through TRPV1 Inhibits Piezo Channels via Phosphoinositide Depletion" @default.
- W2321167647 doi "https://doi.org/10.1016/j.bpj.2014.11.2766" @default.
- W2321167647 hasPublicationYear "2015" @default.
- W2321167647 type Work @default.
- W2321167647 sameAs 2321167647 @default.
- W2321167647 citedByCount "0" @default.
- W2321167647 crossrefType "journal-article" @default.
- W2321167647 hasAuthorship W2321167647A5003816641 @default.
- W2321167647 hasAuthorship W2321167647A5014441183 @default.
- W2321167647 hasAuthorship W2321167647A5068881900 @default.
- W2321167647 hasBestOaLocation W23211676471 @default.
- W2321167647 hasConcept C12554922 @default.
- W2321167647 hasConcept C155164980 @default.
- W2321167647 hasConcept C160145004 @default.
- W2321167647 hasConcept C169760540 @default.
- W2321167647 hasConcept C170493617 @default.
- W2321167647 hasConcept C181104049 @default.
- W2321167647 hasConcept C185592680 @default.
- W2321167647 hasConcept C24998067 @default.
- W2321167647 hasConcept C2778308172 @default.
- W2321167647 hasConcept C2779779143 @default.
- W2321167647 hasConcept C2780051329 @default.
- W2321167647 hasConcept C2780610907 @default.
- W2321167647 hasConcept C2780785647 @default.
- W2321167647 hasConcept C33789571 @default.
- W2321167647 hasConcept C4001165 @default.
- W2321167647 hasConcept C50254741 @default.
- W2321167647 hasConcept C55493867 @default.
- W2321167647 hasConcept C62478195 @default.
- W2321167647 hasConcept C86803240 @default.
- W2321167647 hasConcept C88045685 @default.
- W2321167647 hasConcept C95444343 @default.
- W2321167647 hasConceptScore W2321167647C12554922 @default.
- W2321167647 hasConceptScore W2321167647C155164980 @default.
- W2321167647 hasConceptScore W2321167647C160145004 @default.
- W2321167647 hasConceptScore W2321167647C169760540 @default.
- W2321167647 hasConceptScore W2321167647C170493617 @default.
- W2321167647 hasConceptScore W2321167647C181104049 @default.
- W2321167647 hasConceptScore W2321167647C185592680 @default.
- W2321167647 hasConceptScore W2321167647C24998067 @default.
- W2321167647 hasConceptScore W2321167647C2778308172 @default.
- W2321167647 hasConceptScore W2321167647C2779779143 @default.
- W2321167647 hasConceptScore W2321167647C2780051329 @default.
- W2321167647 hasConceptScore W2321167647C2780610907 @default.
- W2321167647 hasConceptScore W2321167647C2780785647 @default.
- W2321167647 hasConceptScore W2321167647C33789571 @default.
- W2321167647 hasConceptScore W2321167647C4001165 @default.
- W2321167647 hasConceptScore W2321167647C50254741 @default.
- W2321167647 hasConceptScore W2321167647C55493867 @default.
- W2321167647 hasConceptScore W2321167647C62478195 @default.
- W2321167647 hasConceptScore W2321167647C86803240 @default.
- W2321167647 hasConceptScore W2321167647C88045685 @default.
- W2321167647 hasConceptScore W2321167647C95444343 @default.
- W2321167647 hasLocation W23211676471 @default.
- W2321167647 hasOpenAccess W2321167647 @default.
- W2321167647 hasPrimaryLocation W23211676471 @default.
- W2321167647 hasRelatedWork W1591755406 @default.
- W2321167647 hasRelatedWork W1988174555 @default.
- W2321167647 hasRelatedWork W2020204611 @default.
- W2321167647 hasRelatedWork W2030986287 @default.
- W2321167647 hasRelatedWork W2033743296 @default.
- W2321167647 hasRelatedWork W2041339949 @default.
- W2321167647 hasRelatedWork W2048374728 @default.
- W2321167647 hasRelatedWork W2075375255 @default.
- W2321167647 hasRelatedWork W2089809008 @default.
- W2321167647 hasRelatedWork W2136323995 @default.
- W2321167647 hasRelatedWork W2138149759 @default.
- W2321167647 hasRelatedWork W2186295141 @default.
- W2321167647 hasRelatedWork W2554780331 @default.
- W2321167647 hasRelatedWork W2788260932 @default.
- W2321167647 hasRelatedWork W2804642002 @default.
- W2321167647 hasRelatedWork W2898366120 @default.
- W2321167647 hasRelatedWork W2964844997 @default.
- W2321167647 hasRelatedWork W3047976398 @default.
- W2321167647 hasRelatedWork W3168553148 @default.
- W2321167647 hasRelatedWork W3204057746 @default.
- W2321167647 isParatext "false" @default.
- W2321167647 isRetracted "false" @default.
- W2321167647 magId "2321167647" @default.
- W2321167647 workType "article" @default.