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• W2321371738 abstract "The onset of milk production lets mammals experience an enormous energy and nutrient demand. To meet these requirements, high-yielding dairy cows mobilize body fat resulting in an augmented hepatic oxidative metabolism, which has been suggested to signal for depressing hunger after calving. To examine how the extent of fat mobilization influences hepatic oxidative metabolism and thus potentially feed intake, blood and liver samples of 19 Holstein cows were taken throughout the periparturient period. Retrospectively grouped according to high (H) and low (L) liver fat content, H cows showed higher fatty acid but lower amino acid plasma concentrations and lower feed intake than L cows. The hepatic phospho-AMPK/total AMP ratio was not different between groups but decreased after parturition. A 2-DE coupled MALDI-TOF-TOF analysis and qRT-PCR studies revealed H cows having lower expressions of major enzymes involved in mitochondrial β-oxidation, urea cycling, and the pentose phosphate pathway but higher expressions of enzymes participating in peroxisomal and endoplasmic fatty acid degradation, pyruvate and TCA cycling, amino acid catabolism, oxidative phosphorylation, and oxidative stress defense. These data indicate that increasing lipolysis leads to augmenting nutrient catabolism for anaplerosis and mitochondrial respiration, providing a molecular link between hepatic oxidative processes and feed intake." @default.
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• W2321371738 date "2012-10-19" @default.
• W2321371738 modified "2023-10-01" @default.
• W2321371738 title "Increased Anaplerosis, TCA Cycling, and Oxidative Phosphorylation in the Liver of Dairy Cows with Intensive Body Fat Mobilization during Early Lactation" @default.
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• W2321371738 doi "https://doi.org/10.1021/pr300732n" @default.
• W2321371738 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23046364" @default.
• W2321371738 hasPublicationYear "2012" @default.
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