FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2321372005> ?p ?o ?g. }

• W2321372005 endingPage "148" @default.
• W2321372005 startingPage "141" @default.
• W2321372005 abstract "Aim. The obtaining of bioaffinity sorbent based on the immobilized protein A of S. aureus (SPA) using two cellulose-binding domains (CBD), and its application for purification of antibodies. Methods. The DNA sequences encoding SPA and two CBD were genetically fused, expressed in the high-productive Escherichia coli system and the protein SPA-CBD2 was obtained in a soluble form. The SPA-CBD2 fusion protein was affinity immobilized on the microcrystalline cellulose. Results. Capacity of bioaffinity sorbent (1 mg SPA-CBD2/1 ml CC31-cellulose), dynamic capacity (3 mg mouse IgG/1 ml bioaffinity sorbent), efficiency and stability during prolonged storage were determined. The bioffinity sorbent was used for purification of antibodies. The purity of antibodies in eluted fractions was more than 95 %. The purified antibodies detected target antigens with a high sensitivity. Conclusions. The designed bioaffinity sorbent provides obtaining pure polyand monoclonal antibodies in functionally active form and can be useful for the fractionation of mouse immunoglobulin G." @default.
• W2321372005 created "2016-06-24" @default.
• W2321372005 creator A5004188310 @default.
• W2321372005 creator A5029320841 @default.
• W2321372005 creator A5046935925 @default.
• W2321372005 creator A5061119144 @default.
• W2321372005 creator A5070480762 @default.
• W2321372005 date "2012-03-20" @default.
• W2321372005 modified "2023-09-24" @default.
• W2321372005 title "Bioaffinity sorbent based on immobilized protein A Staphylococcus aureus: development and application" @default.
• W2321372005 cites W1933753908 @default.
• W2321372005 cites W2008720195 @default.
• W2321372005 cites W2016775291 @default.
• W2321372005 cites W2029677323 @default.
• W2321372005 cites W2042662079 @default.
• W2321372005 cites W2070393212 @default.
• W2321372005 cites W2077500683 @default.
• W2321372005 cites W2093244155 @default.
• W2321372005 cites W2106246537 @default.
• W2321372005 cites W2120941444 @default.
• W2321372005 cites W2143166347 @default.
• W2321372005 cites W2153489797 @default.
• W2321372005 cites W2331912978 @default.
• W2321372005 cites W2490395854 @default.
• W2321372005 cites W34941183 @default.
• W2321372005 doi "https://doi.org/10.7124/bc.000041" @default.
• W2321372005 hasPublicationYear "2012" @default.
• W2321372005 type Work @default.
• W2321372005 sameAs 2321372005 @default.
• W2321372005 citedByCount "7" @default.
• W2321372005 countsByYear W23213720052013 @default.
• W2321372005 countsByYear W23213720052015 @default.
• W2321372005 countsByYear W23213720052016 @default.
• W2321372005 countsByYear W23213720052017 @default.
• W2321372005 countsByYear W23213720052021 @default.
• W2321372005 countsByYear W23213720052022 @default.
• W2321372005 crossrefType "journal-article" @default.
• W2321372005 hasAuthorship W2321372005A5004188310 @default.
• W2321372005 hasAuthorship W2321372005A5029320841 @default.
• W2321372005 hasAuthorship W2321372005A5046935925 @default.
• W2321372005 hasAuthorship W2321372005A5061119144 @default.
• W2321372005 hasAuthorship W2321372005A5070480762 @default.
• W2321372005 hasBestOaLocation W23213720051 @default.
• W2321372005 hasConcept C104317684 @default.
• W2321372005 hasConcept C150394285 @default.
• W2321372005 hasConcept C159654299 @default.
• W2321372005 hasConcept C178790620 @default.
• W2321372005 hasConcept C179247698 @default.
• W2321372005 hasConcept C181199279 @default.
• W2321372005 hasConcept C185592680 @default.
• W2321372005 hasConcept C203014093 @default.
• W2321372005 hasConcept C2777899863 @default.
• W2321372005 hasConcept C2779000548 @default.
• W2321372005 hasConcept C2779251873 @default.
• W2321372005 hasConcept C2779489039 @default.
• W2321372005 hasConcept C43617362 @default.
• W2321372005 hasConcept C523546767 @default.
• W2321372005 hasConcept C542903549 @default.
• W2321372005 hasConcept C54355233 @default.
• W2321372005 hasConcept C547475151 @default.
• W2321372005 hasConcept C55493867 @default.
• W2321372005 hasConcept C73826308 @default.
• W2321372005 hasConcept C86803240 @default.
• W2321372005 hasConcept C97428945 @default.
• W2321372005 hasConceptScore W2321372005C104317684 @default.
• W2321372005 hasConceptScore W2321372005C150394285 @default.
• W2321372005 hasConceptScore W2321372005C159654299 @default.
• W2321372005 hasConceptScore W2321372005C178790620 @default.
• W2321372005 hasConceptScore W2321372005C179247698 @default.
• W2321372005 hasConceptScore W2321372005C181199279 @default.
• W2321372005 hasConceptScore W2321372005C185592680 @default.
• W2321372005 hasConceptScore W2321372005C203014093 @default.
• W2321372005 hasConceptScore W2321372005C2777899863 @default.
• W2321372005 hasConceptScore W2321372005C2779000548 @default.
• W2321372005 hasConceptScore W2321372005C2779251873 @default.
• W2321372005 hasConceptScore W2321372005C2779489039 @default.
• W2321372005 hasConceptScore W2321372005C43617362 @default.
• W2321372005 hasConceptScore W2321372005C523546767 @default.
• W2321372005 hasConceptScore W2321372005C542903549 @default.
• W2321372005 hasConceptScore W2321372005C54355233 @default.
• W2321372005 hasConceptScore W2321372005C547475151 @default.
• W2321372005 hasConceptScore W2321372005C55493867 @default.
• W2321372005 hasConceptScore W2321372005C73826308 @default.
• W2321372005 hasConceptScore W2321372005C86803240 @default.
• W2321372005 hasConceptScore W2321372005C97428945 @default.
• W2321372005 hasIssue "2" @default.
• W2321372005 hasLocation W23213720051 @default.
• W2321372005 hasOpenAccess W2321372005 @default.
• W2321372005 hasPrimaryLocation W23213720051 @default.
• W2321372005 hasRelatedWork W2011473955 @default.
• W2321372005 hasRelatedWork W2020163727 @default.
• W2321372005 hasRelatedWork W2023602932 @default.
• W2321372005 hasRelatedWork W2047621215 @default.
• W2321372005 hasRelatedWork W2063886143 @default.
• W2321372005 hasRelatedWork W2078699270 @default.
• W2321372005 hasRelatedWork W2082480737 @default.
• W2321372005 hasRelatedWork W2999122680 @default.
• W2321372005 hasRelatedWork W4281703794 @default.

• next