Matches in SemOpenAlex for { <https://semopenalex.org/work/W232138214> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W232138214 abstract "Abstract : The activities of the Children's Hospital of Pittsburgh Histocompatibility Center can be classified under three major categories: 1) the repository service; 2) the molecular typing service; and 3) the research activities aimed at improving the technical support on which the previous two services are working. This year in the Repository, where blood samples from volunteers as bone marrow donors are collected from more than 125 recruiting centers nationwide, 165,203 samples were processed. We stored 495,609 vials in three sets of freezers (one sample per donor each freezer), while 216,712 were sent out to other Typing Laboratories for molecular HLA typings. In our laboratories, 10,626 typings were performed with a very high Q.C. standard, as monitored by National Marrow Donor Program. Our Center's research branch synthesized all the oligonucleotides to be used as PCR primers or labelled probes to perform molecular HLA typing. DNA sequencing was also performed when hybridization results were not optimal. Alternatives for storing blood samples (e.g., blood spots on filter paper) or for enhancing typing capabilities (e.g., microchip technology to expedite PCR procedures) were successfully implemented or improved to the point of justifying their future implementation." @default.
- W232138214 created "2016-06-24" @default.
- W232138214 creator A5017682705 @default.
- W232138214 date "1999-12-16" @default.
- W232138214 modified "2023-09-28" @default.
- W232138214 title "Children's Hospital of Pittsburgh Histocompatibility Center Nucleotide Sequencing for Detection of Alleles at HLA Class I and Class II Loci on Ethnic Minorities" @default.
- W232138214 doi "https://doi.org/10.21236/ada371509" @default.
- W232138214 hasPublicationYear "1999" @default.
- W232138214 type Work @default.
- W232138214 sameAs 232138214 @default.
- W232138214 citedByCount "0" @default.
- W232138214 crossrefType "report" @default.
- W232138214 hasAuthorship W232138214A5017682705 @default.
- W232138214 hasConcept C129312508 @default.
- W232138214 hasConcept C147483822 @default.
- W232138214 hasConcept C188280979 @default.
- W232138214 hasConcept C202965653 @default.
- W232138214 hasConcept C2781209916 @default.
- W232138214 hasConcept C2909478873 @default.
- W232138214 hasConcept C54355233 @default.
- W232138214 hasConcept C552990157 @default.
- W232138214 hasConcept C71924100 @default.
- W232138214 hasConcept C86803240 @default.
- W232138214 hasConceptScore W232138214C129312508 @default.
- W232138214 hasConceptScore W232138214C147483822 @default.
- W232138214 hasConceptScore W232138214C188280979 @default.
- W232138214 hasConceptScore W232138214C202965653 @default.
- W232138214 hasConceptScore W232138214C2781209916 @default.
- W232138214 hasConceptScore W232138214C2909478873 @default.
- W232138214 hasConceptScore W232138214C54355233 @default.
- W232138214 hasConceptScore W232138214C552990157 @default.
- W232138214 hasConceptScore W232138214C71924100 @default.
- W232138214 hasConceptScore W232138214C86803240 @default.
- W232138214 hasLocation W2321382141 @default.
- W232138214 hasOpenAccess W232138214 @default.
- W232138214 hasPrimaryLocation W2321382141 @default.
- W232138214 hasRelatedWork W135465708 @default.
- W232138214 hasRelatedWork W1973229613 @default.
- W232138214 hasRelatedWork W1987179920 @default.
- W232138214 hasRelatedWork W1991493200 @default.
- W232138214 hasRelatedWork W2007170029 @default.
- W232138214 hasRelatedWork W2014797413 @default.
- W232138214 hasRelatedWork W2023557010 @default.
- W232138214 hasRelatedWork W2024490411 @default.
- W232138214 hasRelatedWork W2031408141 @default.
- W232138214 hasRelatedWork W2048207676 @default.
- W232138214 hasRelatedWork W2053815063 @default.
- W232138214 hasRelatedWork W2071531342 @default.
- W232138214 hasRelatedWork W2105388580 @default.
- W232138214 hasRelatedWork W2169024552 @default.
- W232138214 hasRelatedWork W2183754789 @default.
- W232138214 hasRelatedWork W2206906606 @default.
- W232138214 hasRelatedWork W2298455375 @default.
- W232138214 hasRelatedWork W2468183768 @default.
- W232138214 hasRelatedWork W2743765368 @default.
- W232138214 hasRelatedWork W2750798548 @default.
- W232138214 isParatext "false" @default.
- W232138214 isRetracted "false" @default.
- W232138214 magId "232138214" @default.
- W232138214 workType "report" @default.