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• W2321483575 abstract "Three cryoprotectant solvents, dimethylsulphoxide, 1,2-propanediol and glycerol, were investigated for a non-enzymatic reaction product, formaldehyde. All three cryoprotectants demonstrated a direct relationship between increasing solvent molarity and increasing formaldehyde concentration which was independent of temperature and protein (bovine serum albumin). Medium composition significantly influenced the formaldehyde concentration with HTF ≥ T6 ≥ M16 = M2. The formaldehyde could be effectively removed by reduced glutathione, cysteine and dithiothreitol with cysteine being the most effective scavenging agent A reaction mechanism for this scavenging is proposed. The combination of cysteine and cryoprotectant reduced the zona pellucida ‘hardening’ effect in mouse oocytes." @default.
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• W2321483575 date "1996-12-01" @default.
• W2321483575 modified "2023-10-18" @default.
• W2321483575 title "Fertilization and early embryology: Non-enzymatic formation of formaldehyde in mouse oocyte freezing mixtures" @default.
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• W2321483575 doi "https://doi.org/10.1093/oxfordjournals.humrep.a019191" @default.
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