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- W2321551319 abstract "AbstractThe objective of this study was to evaluate the production of pectinase by an isolated strain of Penicillium brasilianum in a bioreactor and to consider its potential for industrial applications (i.e. fruit juice). The optimization of production was achieved through experimental design. The maximum exo-polygalacturonase (Exo-PG) production in the bioreactor was 53.8 U mL−1 under the conditions of 180 rpm, an aeration rate of 1.5 vvm, 30 °C, pHinitial of 5.5, 5 × 106 spores mL−1, 32 g L−1 pectin, 10 g L−1 of yeast extract and 0.5 g L−1 magnesium sulfate and bioproduction for 36 h. The production of Exo-PG in the bioreactor was 1.3 times higher than that obtained in shake flasks, with aeration (1.5 vvm) and agitation (180 rpm) control. The crude enzyme complex, beyond the pectinolytic activity of Exo-PG (53.8 U mL−1), also contained activity pectin methylesterase (6.0 U mL−1) and pectin lyase (6.61 U mL−1). At a crude enzyme complex with a concentration of 0.5% (v/v), viscosity of peach juice was re..." @default.
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- W2321551319 date "2015-11-02" @default.
- W2321551319 modified "2023-09-24" @default.
- W2321551319 title "Production and characterization ofPenicillium brasilianumpectinases with regard to industrial application" @default.
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- W2321551319 doi "https://doi.org/10.3109/10242422.2016.1162788" @default.
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