FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2321553901> ?p ?o ?g. }

• W2321553901 abstract "Event Abstract Back to Event Efficacy of antimicrobial impregnated urinary catheter in thwarting foreign body associated urinary tract infections caused by Pseudomonas aeruginosa Hina Saini1, Sanjay Chhibber1 and Kusum Harjai1 1 Panjab University, Microbiology, India Introduction: Catheter associated urinary tract infections (CAUTIs) are rising exponentially around the globe; especially in intensive care units. The formation of biofilm on the surface of an indwelling urinary catheter (UC) marks the onset of CAUTI. Treatment of CAUTI/eradication of biofilm poses an intricate challenge to clinicians and researchers since bacteria within biofilm have increased protection against host immune response and resistance to antimicrobials. Biofilm-associated bacteria on the surface of UC produce quorum sensing (QS) signal molecules which regulate the expression of genes required for biofilm formation. Attenuation of QS, which in turn regulates biofilm production, seems to be a novel approach to curb CAUTI. The present study was thus designed with a preventive strategy to generate a biomaterial by impregnating a silicone UC with combination of azithromycin (AZM) and ciprofloxacin (CIP) and testing its efficacy in-vitro against Pseudomonas aeruginosa. Materials and Methods: Medical grade silicone tubing (0.30mm X 0.64mm) was impregnated with antimicrobials: 3% CIP and 6% AZM. In-vitro efficacy was assessed for control and AZM-CIP impregnated UC against P. aeruginosa standard strain PAO1 using serial plate transfer test (SPTT), in-vitro biofilm colonization, durability test, scanning electron microscopy (SEM) to visualise biofilm and shelf-life test. Results and Discussion: SPTT of AZM-CIP impregnated UC revealed prominent zones of inhibition (> 30mm) against P. aeruginosa PAO1 during testing period of one month and no bacterial growth was observed around or under the UC. These results indicate prolonged antimicrobial activity of these catheters with sustained diffusion of drugs and absence of resistance development since no bacteria was found attached to catheters following continuous exposure to heavy inoculum of P. aeruginosa. AZM-CIP impregnated UC showed complete inhibition of bacterial colonization and biofilm formation after 24h exposure to P. aeruginosa PAO1. These findings suggest anti-adherence activity of catheters. AZM-CIP impregnated UC were tested for durability by dipping in sterile human urine for 4 weeks simulating biological conditions. The results showed significant decline in biofilm formation over 4 weeks suggesting longer durability. These findings were further confirmed by SEM which showed limited bacterial cells within disrupted biofilm on the surface of antimicrobial impregnated UC as compared with fully grown intact biofilm on the surface of control catheters. Additionally, AZM-CIP impregnated UC showed complete inhibition of biofilm formation at quarterly intervals displaying stable real-time shelf life for one year. Conclusion: AZM-CIP impregnated UC demonstrated prolonged duration of antimicrobial and antibiofilm activity. The present study bring out the fact that synergistic combination of currently available antibiotics AZM and CIP may be usefully applied to generate a biomaterial by impregnating these drugs into a UC to prevent bacterial colonization and biofilm formation. Such a catheter would be beneficial in clinical situations, especially in long-term foreign body associated urinary tract infections. This work was funded by University Grants Commission (UGC), New Delhi, India (F1-17.1/2013-14/MANF-2013-14-SIK-CHA-22060/(SA-III/Website). We thank Sophisticated Analytical Instrumentation Facility (SAIF), Panjab University, Chandigarh for their kind support in Scanning Electron MicroscopyReferences:[1] Hanna H, Bahna P, Reitzel R, Dvorak T, Chaiban G, Hachem R, Raad I. 2006. Comparative in vitro efficacies and antimicrobial durabilities of novel antimicrobial central venous catheters. Antimicrobial Agents and Chemotherapy 50(10): 3283-3288.[2] Raad I, Mohamed JA, Reitzel R, Jiang Y, Raad S, Al Shuaibi M, Chaftari A-M, Hachem R. 2012. Improved antibiotic-impregnated catheters with extended-spectrum activity against resistant bacteria and fungi. Antimicrobial Agents and Chemotherapy. 56(2): 935-941.[3] Saini H, Chhibber S, Harjai K. 2015. Azithromycin and ciprofloxacin: A possible synergistic combination against Pseudomonas aeruginosa biofilm-associated urinary tract infections. International Journal of Antimicrobial Agents. 45(4): 359-367. Keywords: Bacteria, in vitro, drug, biomaterial Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: General Session Oral Topic: Anti-infective biomaterials Citation: Saini H, Chhibber S and Harjai K (2016). Efficacy of antimicrobial impregnated urinary catheter in thwarting foreign body associated urinary tract infections caused by Pseudomonas aeruginosa. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.01647 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Hina Saini Sanjay Chhibber Kusum Harjai Google Hina Saini Sanjay Chhibber Kusum Harjai Google Scholar Hina Saini Sanjay Chhibber Kusum Harjai PubMed Hina Saini Sanjay Chhibber Kusum Harjai Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
• W2321553901 created "2016-06-24" @default.
• W2321553901 creator A5033113014 @default.
• W2321553901 creator A5040604872 @default.
• W2321553901 creator A5075709522 @default.
• W2321553901 date "2016-01-01" @default.
• W2321553901 modified "2023-09-27" @default.
• W2321553901 title "Efficacy of antimicrobial impregnated urinary catheter in thwarting foreign body associated urinary tract infections caused by Pseudomonas aeruginosa" @default.
• W2321553901 doi "https://doi.org/10.3389/conf.fbioe.2016.01.01647" @default.
• W2321553901 hasPublicationYear "2016" @default.
• W2321553901 type Work @default.
• W2321553901 sameAs 2321553901 @default.
• W2321553901 citedByCount "0" @default.
• W2321553901 crossrefType "journal-article" @default.
• W2321553901 hasAuthorship W2321553901A5033113014 @default.
• W2321553901 hasAuthorship W2321553901A5040604872 @default.
• W2321553901 hasAuthorship W2321553901A5075709522 @default.
• W2321553901 hasBestOaLocation W23215539011 @default.
• W2321553901 hasConcept C118687296 @default.
• W2321553901 hasConcept C126322002 @default.
• W2321553901 hasConcept C178790620 @default.
• W2321553901 hasConcept C185592680 @default.
• W2321553901 hasConcept C2777637488 @default.
• W2321553901 hasConcept C2778512257 @default.
• W2321553901 hasConcept C2779769944 @default.
• W2321553901 hasConcept C4937899 @default.
• W2321553901 hasConcept C501593827 @default.
• W2321553901 hasConcept C523546767 @default.
• W2321553901 hasConcept C54355233 @default.
• W2321553901 hasConcept C58123911 @default.
• W2321553901 hasConcept C71924100 @default.
• W2321553901 hasConcept C77411442 @default.
• W2321553901 hasConcept C86803240 @default.
• W2321553901 hasConcept C89423630 @default.
• W2321553901 hasConceptScore W2321553901C118687296 @default.
• W2321553901 hasConceptScore W2321553901C126322002 @default.
• W2321553901 hasConceptScore W2321553901C178790620 @default.
• W2321553901 hasConceptScore W2321553901C185592680 @default.
• W2321553901 hasConceptScore W2321553901C2777637488 @default.
• W2321553901 hasConceptScore W2321553901C2778512257 @default.
• W2321553901 hasConceptScore W2321553901C2779769944 @default.
• W2321553901 hasConceptScore W2321553901C4937899 @default.
• W2321553901 hasConceptScore W2321553901C501593827 @default.
• W2321553901 hasConceptScore W2321553901C523546767 @default.
• W2321553901 hasConceptScore W2321553901C54355233 @default.
• W2321553901 hasConceptScore W2321553901C58123911 @default.
• W2321553901 hasConceptScore W2321553901C71924100 @default.
• W2321553901 hasConceptScore W2321553901C77411442 @default.
• W2321553901 hasConceptScore W2321553901C86803240 @default.
• W2321553901 hasConceptScore W2321553901C89423630 @default.
• W2321553901 hasLocation W23215539011 @default.
• W2321553901 hasOpenAccess W2321553901 @default.
• W2321553901 hasPrimaryLocation W23215539011 @default.
• W2321553901 hasRelatedWork W1968602861 @default.
• W2321553901 hasRelatedWork W2073717190 @default.
• W2321553901 hasRelatedWork W2075859309 @default.
• W2321553901 hasRelatedWork W2129197708 @default.
• W2321553901 hasRelatedWork W2500673925 @default.
• W2321553901 hasRelatedWork W2918569058 @default.
• W2321553901 hasRelatedWork W3094496711 @default.
• W2321553901 hasRelatedWork W3097161563 @default.
• W2321553901 hasRelatedWork W3097647920 @default.
• W2321553901 hasRelatedWork W3199170223 @default.
• W2321553901 hasVolume "4" @default.
• W2321553901 isParatext "false" @default.
• W2321553901 isRetracted "false" @default.
• W2321553901 magId "2321553901" @default.
• W2321553901 workType "article" @default.