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- W2321813948 abstract "Combined chromatin immunoprecipitation and next generation sequencing (ChIP-seq) has become an extremely popular method to generate genome-wide epigenetic profiles from numerous cell lines and tissue types. Typical ChIP-seq experiments require large number of cells, making them illadapted to the study of rare cell populations. This procedure describes an ultra-low-input (ULI) micrococcal nuclease-based native ChIP (NChIP) and sequencing library construction method to generate genome-wide chromatin profiles from as few as 10 cells (Brind’Amour et al., 10.1038/ncomms7033). In addition, ULI-NChIP-seq has been validated in vivo, by generation of H3K9me3 and H3K27me3 profiles from E13.5 primordial germ cells isolated from single embryos (Liu, Brind’Amour et al., 10.1101/gad.244848.114)." @default.
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- W2321813948 date "2015-01-27" @default.
- W2321813948 modified "2023-10-16" @default.
- W2321813948 title "Ultra-low-input native ChIP-seq for rare cell populations" @default.
- W2321813948 doi "https://doi.org/10.1038/protex.2015.007" @default.
- W2321813948 hasPublicationYear "2015" @default.
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