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- W2321833566 abstract "A rapid radioimmunoassay (RIA) method for serum estradiol using directly radioiodinated estradiol and polyethylene glycol was devised. Estradiol (E2) was directly radioiodinated by the chloramine T method with minor modifications and purified with two dimensional thin layer chromatography. After mixing the standard or sample solution, antibody solution, and radioiodine labelled estradiol solution, the mixture was incubated for 15 minutes at 37°C. Polyethylene glycol was used for the separation of the bound and unbound form. Estradiol was serially diluted with the steroid free serum which was prepared from non-pregnant women serum by the dextran coated charcoal adsorption to be used for “serum standard” for evaluating unknown samples. The intraassay and interassay coefficient of variations were 8.6% and 10.3% respectively. It took only 2 to 3 hours to determine the serum E2 values. This method seems to be useful for serial monitoring of induction of ovulation by human menopausal gonadotropin and other medical practice in rapidity, simplicity and disposability." @default.
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- W2321833566 date "1983-01-01" @default.
- W2321833566 modified "2023-09-27" @default.
- W2321833566 title "A NEW METHOD OF RADIOIODINATION OF THE ESTRADIOL (E2) AND RAPID RADIOIMMUNOASSAY OF SERUM E2" @default.
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- W2321833566 doi "https://doi.org/10.2974/kmj1951.33.409" @default.
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