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• W2321833860 abstract "MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections MEPS 450:275-280 (2012) - DOI: https://doi.org/10.3354/meps09513 NOTE Temperature-induced microbubbles within natural marine samples may inflate small-particle counts in a Coulter Counter Edward J. Rice, Caterina Panzeca, Gillian M. Stewart* The School of Earth and Environmental Sciences, Queens College, City University of New York (CUNY), Flushing, New York 11367, USA *Corresponding author. Email: gstewart@qc.cuny.edu ABSTRACT: The Coulter Counter, a common instrument used to enumerate phytoplankton, may over-estimate counts of particles <2.5 µm in equivalent spherical diameter (ESD) by an order of magnitude when samples are run at temperatures cooler than ambient laboratory conditions. This phenomenon is likely due to microbubbles generated as a colder sample warms. Evidence for this mechanism derives from the observation that increasing the relative fraction of organic-rich coastal water in warming samples results in increased amplification of small-particle counts due to the stabilization of microbubbles. Count amplification can be eliminated by ensuring there is no temperature difference between the diluent and the sample. Failing to correct for this error confounds analysis of marine phytoplankton size spectra, complicating a broad range of experiments from those measuring productivity to those used to develop ecosystem-based models. KEY WORDS: Coulter Counter · Microbubbles · Temperature · Particle size · Picoplankton Full text in pdf format PreviousNextCite this article as: Rice EJ, Panzeca C, Stewart GM (2012) Temperature-induced microbubbles within natural marine samples may inflate small-particle counts in a Coulter Counter. Mar Ecol Prog Ser 450:275-280. https://doi.org/10.3354/meps09513 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in MEPS Vol. 450. Online publication date: March 29, 2012 Print ISSN: 0171-8630; Online ISSN: 1616-1599 Copyright © 2012 Inter-Research." @default.
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• W2321833860 title "Temperature-induced microbubbles within natural marine samples may inflate small-particle counts in a Coulter Counter" @default.
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• W2321833860 doi "https://doi.org/10.3354/meps09513" @default.
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