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- W2321868547 abstract "The bone marrow microenvironment maintains a stable balance between self-renewal and differentiation of hematopoietic stem/progenitor cells (HSPCs). This microenvironment, also termed the “hematopoietic niche”, is primarily composed of stromal cells and their extracellular matrices (ECM) that jointly regulate HSPC functions. Previously, we have demonstrated that umbilical cord blood derived HSPCs can be maintained and expanded on stromal cell derived cellular matrices that mimic the complexity of the hematopoietic niche. The results indicated that matrices prepared at 20% O2 with osteogenic medium (OGM) were best suited for expanding committed HSPCs, whereas, matrices prepared at 5% O2 without OGM were better for primitive progenitors. Based upon these results we proposed that individual constituents of these matrices could be responsible for regulation of specific HSPC functions. To explore this hypothesis, we have performed comparative transcriptome profiling of these matrix producing cells, which identified differential expression of both known niche regulators, such as Wnt4, Angpt2, Vcam and Cxcl12, as well as genes not previously associated with HSPC regulation, such as Depp. MetaCore analysis of the differentially expressed genes suggests the down-regulation of several ECM related pathways and up-regulation of Ang-Tie2 and Wnt signaling pathways in OGM under high O2 (20%). Our findings provide an overview of several known and unique genes and pathways that play potential key roles in the support of HSPCs by stromal cells, both ex vivo and in vivo, and could be helpful in understanding the complex network of signaling and communication in hematopoietic niches." @default.
- W2321868547 created "2016-06-24" @default.
- W2321868547 creator A5074577965 @default.
- W2321868547 date "2013-01-01" @default.
- W2321868547 modified "2023-09-25" @default.
- W2321868547 title "Comparative Gene Expression Profiling of Stromal Cell Matrices that Support Expansion of Hematopoietic Stem/Progenitor Cells" @default.
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- W2321868547 doi "https://doi.org/10.4172/2157-7633.1000152" @default.
- W2321868547 hasPublicationYear "2013" @default.