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- W2321869745 abstract "AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials AME 68:43-55 (2012) - DOI: https://doi.org/10.3354/ame01600 Light:dark (12:12 h) quantification of carbohydrate fluxes in Crocosphaera watsonii Anthony Dron1,2, Sophie Rabouille1,2,*, Pascal Claquin4,5, Patrick Chang1,3, Virginie Raimbault4,5, Amélie Talec1,2, Antoine Sciandra1,2 1UPMC Univ Paris 06, UMR 7093, LOV, Observatoire océanologique, 06234, Villefranche-sur-mer, France 2CNRS, UMR 7093, LOV, Observatoire océanologique, 06234, Villefranche-sur-mer, France 3CNRS, UMR 7009, Biologie du développement, 06230, Villefranche-sur-mer, France 4Université Caen Basse-Normandie, FRE BIOMEA, 14032 Caen, France 5CNRS INEE, FRE BIOMEA, 14032 Caen, France *Email: srabouille@obs-vlfr.fr ABSTRACT: Light:dark (12:12 h) quantification of cellular carbohydrate fluxes was conducted with the unicellular, N2-fixing cyanobacterium Crocosphaera watsonii WH8501 grown in continuous cultures under conditions of diazotrophy. As commonly observed in photoautotrophs, C. watsonii accumulates photosynthetic products as carbohydrates, which thereafter serve as an internal energy reserve for energy-demanding processes. Our goal was to further discriminate between cellular pools with different sizes to observe their respective dynamics. Total cellular carbohydrate as well as the cellular carbohydrate pools showed a diel cycle of accumulation and decrease. Total cellular carbohydrate contents represented 45 and 32% of the cellular carbon content at the end of the light and dark period, respectively. The observed decrease in cellular carbohydrate was not only due to carbohydrate catabolism but also to their release in the environment as extracellular polymeric substances (EPS), including soluble EPS and transparent exopolymeric particles (TEP). The occurrence of such excretion during the exponential growth of C. watsonii appears as part of the metabolic regulations that participate in maintaining a daily carbon:nitrogen balanced growth. About 10% of the total carbon cell content is excreted daily as soluble EPS. TEP, which are formed from dissolved precursors (including some soluble EPS) released by C. watsonii, increased during the dark period, when they represented up to 3.6% of total carbon cell content. Such recurrent release of carbohydrate by populations of C. watsonii is expected to stimulate the microbial loop in warm, oligotrophic oceans. KEY WORDS: Carbon · Extracellular polymeric substances · EPS · Transparent exopolymeric particles · TEP · Diazotroph · UCYN · Continuous culture · Excretion Full text in pdf format PreviousNextCite this article as: Dron A, Rabouille S, Claquin P, Chang P, Raimbault V, Talec A, Sciandra A (2012) Light:dark (12:12 h) quantification of carbohydrate fluxes in Crocosphaera watsonii . Aquat Microb Ecol 68:43-55. https://doi.org/10.3354/ame01600 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AME Vol. 68, No. 1. Online publication date: November 16, 2012 Print ISSN: 0948-3055; Online ISSN: 1616-1564 Copyright © 2012 Inter-Research." @default.
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- W2321869745 title "Light:dark (12:12 h) quantification of carbohydrate fluxes in Crocosphaera watsonii " @default.
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