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• W2321876647 abstract "Although structural DNA nanotechnology, and especially scaffolded DNA origami, hold great promise for bottom-up fabrication of novel nanoscale materials and devices, concerns about scalability have tempered widespread enthusiasm. Here we report a single-pot reaction where both strands of double-stranded M13-bacteriophage DNA are simultaneously folded into two distinct shapes that then heterodimerize with high yield. The fully addressable, two-dimensional heterodimer DNA origami, with twice the surface area of standard M13 origami, formed in high yield (81% of the well-formed monomers undergo dimerization). We also report the concurrent production of entire sets of staple strands by a unique, nicking strand-displacement amplification (nSDA) involving reusable surface-bound template strands that were synthesized in situ using a custom piezoelectric inkjet system. The combination of chip-based staple strand production, double-sized origami, and high-yield one-pot assembly markedly increases the useful scale of DNA origami." @default.
• W2321876647 created "2016-06-24" @default.
• W2321876647 creator A5010196721 @default.
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• W2321876647 date "2013-01-09" @default.
• W2321876647 modified "2023-09-26" @default.
• W2321876647 title "One-Pot Assembly of a Hetero-dimeric DNA Origami from Chip-Derived Staples and Double-Stranded Scaffold" @default.
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• W2321876647 doi "https://doi.org/10.1021/nn302322j" @default.
• W2321876647 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23281627" @default.
• W2321876647 hasPublicationYear "2013" @default.
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