Matches in SemOpenAlex for { <https://semopenalex.org/work/W2321879847> ?p ?o ?g. }
- W2321879847 endingPage "83" @default.
- W2321879847 startingPage "77" @default.
- W2321879847 abstract "Alkaline α-amylase from Bacillus sp. strain KSM-K38 (AmyK38) is a calcium-free enzyme that is stable against chelating and oxidative reagents. Recently, the thermostability of this enzyme was improved by replacement of its amino-terminal 11 amino acid residues with the corresponding residues of α-amylase from Bacillus sp. strain KSM-1378. In this study, to further improve the thermal stability, we compared the three-dimensional structure of AmyK38 with that of a hyper-thermostable α-amylase from Bacillus licheniformis. Using site-directed mutagenesis, we created a new possible ionic interaction in the flexible loop region from Gln167 to Gln170 of AmyK38 because the corresponding region in α-amylase from B. licheniformis has an ionic interaction between Glu167 and Lys170. Substitution of Gln167 or Tyr169 with Glu or Lys, respectively, was found to enhance the thermostability of AmyK38. Combination of both substitutions with replacement of the amino-terminal 11 residues further improved the thermostability of the enzyme." @default.
- W2321879847 created "2016-06-24" @default.
- W2321879847 creator A5001302260 @default.
- W2321879847 creator A5001666426 @default.
- W2321879847 creator A5027286765 @default.
- W2321879847 creator A5042487885 @default.
- W2321879847 creator A5042922418 @default.
- W2321879847 creator A5058338752 @default.
- W2321879847 creator A5058999827 @default.
- W2321879847 creator A5060462910 @default.
- W2321879847 creator A5070058874 @default.
- W2321879847 date "2007-01-01" @default.
- W2321879847 modified "2023-10-16" @default.
- W2321879847 title "Improvement of the Thermal Stability of a Calcium-free, Alkaline .ALPHA.-Amylase by Site-directed Mutagenesis" @default.
- W2321879847 cites W1549709155 @default.
- W2321879847 cites W1813790512 @default.
- W2321879847 cites W1988883176 @default.
- W2321879847 cites W1990444791 @default.
- W2321879847 cites W1998591058 @default.
- W2321879847 cites W1998869143 @default.
- W2321879847 cites W2035283686 @default.
- W2321879847 cites W2059297512 @default.
- W2321879847 cites W2093960125 @default.
- W2321879847 cites W2102948189 @default.
- W2321879847 cites W2133719735 @default.
- W2321879847 cites W2142213163 @default.
- W2321879847 cites W25941542 @default.
- W2321879847 doi "https://doi.org/10.5458/jag.54.77" @default.
- W2321879847 hasPublicationYear "2007" @default.
- W2321879847 type Work @default.
- W2321879847 sameAs 2321879847 @default.
- W2321879847 citedByCount "7" @default.
- W2321879847 countsByYear W23218798472012 @default.
- W2321879847 countsByYear W23218798472013 @default.
- W2321879847 countsByYear W23218798472022 @default.
- W2321879847 countsByYear W23218798472023 @default.
- W2321879847 crossrefType "journal-article" @default.
- W2321879847 hasAuthorship W2321879847A5001302260 @default.
- W2321879847 hasAuthorship W2321879847A5001666426 @default.
- W2321879847 hasAuthorship W2321879847A5027286765 @default.
- W2321879847 hasAuthorship W2321879847A5042487885 @default.
- W2321879847 hasAuthorship W2321879847A5042922418 @default.
- W2321879847 hasAuthorship W2321879847A5058338752 @default.
- W2321879847 hasAuthorship W2321879847A5058999827 @default.
- W2321879847 hasAuthorship W2321879847A5060462910 @default.
- W2321879847 hasAuthorship W2321879847A5070058874 @default.
- W2321879847 hasBestOaLocation W23218798471 @default.
- W2321879847 hasConcept C103408237 @default.
- W2321879847 hasConcept C104317684 @default.
- W2321879847 hasConcept C105702510 @default.
- W2321879847 hasConcept C143065580 @default.
- W2321879847 hasConcept C16318435 @default.
- W2321879847 hasConcept C170835558 @default.
- W2321879847 hasConcept C178790620 @default.
- W2321879847 hasConcept C181199279 @default.
- W2321879847 hasConcept C185592680 @default.
- W2321879847 hasConcept C2776527165 @default.
- W2321879847 hasConcept C2777272437 @default.
- W2321879847 hasConcept C2778022156 @default.
- W2321879847 hasConcept C2781216036 @default.
- W2321879847 hasConcept C501734568 @default.
- W2321879847 hasConcept C523546767 @default.
- W2321879847 hasConcept C54355233 @default.
- W2321879847 hasConcept C55493867 @default.
- W2321879847 hasConcept C59061564 @default.
- W2321879847 hasConcept C71240020 @default.
- W2321879847 hasConcept C86803240 @default.
- W2321879847 hasConceptScore W2321879847C103408237 @default.
- W2321879847 hasConceptScore W2321879847C104317684 @default.
- W2321879847 hasConceptScore W2321879847C105702510 @default.
- W2321879847 hasConceptScore W2321879847C143065580 @default.
- W2321879847 hasConceptScore W2321879847C16318435 @default.
- W2321879847 hasConceptScore W2321879847C170835558 @default.
- W2321879847 hasConceptScore W2321879847C178790620 @default.
- W2321879847 hasConceptScore W2321879847C181199279 @default.
- W2321879847 hasConceptScore W2321879847C185592680 @default.
- W2321879847 hasConceptScore W2321879847C2776527165 @default.
- W2321879847 hasConceptScore W2321879847C2777272437 @default.
- W2321879847 hasConceptScore W2321879847C2778022156 @default.
- W2321879847 hasConceptScore W2321879847C2781216036 @default.
- W2321879847 hasConceptScore W2321879847C501734568 @default.
- W2321879847 hasConceptScore W2321879847C523546767 @default.
- W2321879847 hasConceptScore W2321879847C54355233 @default.
- W2321879847 hasConceptScore W2321879847C55493867 @default.
- W2321879847 hasConceptScore W2321879847C59061564 @default.
- W2321879847 hasConceptScore W2321879847C71240020 @default.
- W2321879847 hasConceptScore W2321879847C86803240 @default.
- W2321879847 hasIssue "2" @default.
- W2321879847 hasLocation W23218798471 @default.
- W2321879847 hasOpenAccess W2321879847 @default.
- W2321879847 hasPrimaryLocation W23218798471 @default.
- W2321879847 hasRelatedWork W1523647316 @default.
- W2321879847 hasRelatedWork W1974604210 @default.
- W2321879847 hasRelatedWork W1977356696 @default.
- W2321879847 hasRelatedWork W1992222215 @default.
- W2321879847 hasRelatedWork W2070641276 @default.
- W2321879847 hasRelatedWork W2138435059 @default.
- W2321879847 hasRelatedWork W2151732882 @default.