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- W2321899330 endingPage "2334" @default.
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- W2321899330 abstract "Pyridoxal-5′-phosphate (PLP) represents an active form of Vitamin B6 that shows relatively fast imine formation with hydrazines under physiological conditions without the need of a catalyst. A convenient phosphate/amine conjugation protocol was developed to covalently link PLP to proteins, affording proteins capable of hydrazone formation with bioorthogonal hydrazinyl functional groups. Thus, the lectin Concanavalin A (Con A) was labeled with PLP. Pretreatment with fluorescein hydrazide gave dye-labeled Con A that labeled cell surfaces efficiently. Alternatively, pretargeting was achieved by labeling cells with Con A-PLP, then treatment in vitro with Alexa Fluor 488 hydrazide." @default.
- W2321899330 created "2016-06-24" @default.
- W2321899330 creator A5002521020 @default.
- W2321899330 creator A5044201114 @default.
- W2321899330 date "2012-12-03" @default.
- W2321899330 modified "2023-10-15" @default.
- W2321899330 title "Rapid Catalyst-Free Hydrazone Ligation: Protein-Pyridoxal Phosphoramides" @default.
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- W2321899330 doi "https://doi.org/10.1021/bc300430k" @default.
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