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• W2321899531 abstract "In Brief OBJECTIVE: To estimate whether potential clinical applications of cell-free fetal and total DNA in the field of noninvasive prenatal diagnosis need to be adjusted for maternal smoking status. METHODS: In this study, using 344 maternal blood samples from the second trimester of pregnancy, circulating cell-free DNA in maternal plasma samples, specific for the SRY and DYS14 loci (representing fetal DNA) and GAPDH sequence (representing total genomic DNA) were quantified by real-time polymerase chain reaction. RESULTS: Fetal sex determination was 100% accurate using a combination of probes for SRY and DYS14. The levels of DYS14 and SRY detected were significantly correlated (r=0.884, P<.001). No significant difference was seen between the quantitative levels of cell-free male fetal DNA between the smoking groups and control group. Similarly, no significant difference was seen in the amount of total cell-free DNA in the study population. CONCLUSION: In contrast to first- and second-trimester screening assays for Down syndrome, where smoking status significantly affect levels of maternal serum analytes, smoking status does not affect quantitative levels of cell-free fetal DNA or total cell-free DNA in maternal plasma. LEVEL OF EVIDENCE: II Maternal smoking status does not affect quantitative levels of cell-free fetal and total DNA levels in maternal plasma from the second trimester of pregnancy." @default.
• W2321899531 created "2016-06-24" @default.
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• W2321899531 date "2007-12-01" @default.
• W2321899531 modified "2023-10-05" @default.
• W2321899531 title "Maternal Smoking" @default.
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• W2321899531 doi "https://doi.org/10.1097/01.aog.0000295654.55971.9f" @default.
• W2321899531 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18055732" @default.
• W2321899531 hasPublicationYear "2007" @default.
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