FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2322070115> ?p ?o ?g. }

• W2322070115 endingPage "448" @default.
• W2322070115 startingPage "443" @default.
• W2322070115 abstract "Expansion of haemopoietic stem cells from placental blood has been obtained with a combination of flt3 ligand (FL), thrombopoietin (TPO), kit-ligand (KL) with or without interleukin-6 (IL6) in serum-replete medium. For clinical use, cell expansion in the absence of serum is a clear advantage. Therefore, stem cell expansion in serum-free (SF) medium with a combination of three (FL, TPO, KL) or four (FL, TPO, KL, IL6) growth factors was compared with the results obtained using fetal calf serum (FCS) or human serum (HS). Human CD34(+) placental blood cells were cultured in the presence of FL, TPO, KL +/- IL6 with SF medium, HS and FCS for up to 8 weeks. CD34(+), CFC, LTC-IC content was measured at intervals. To determine the in vivo repopulating capacity of expanded cells, CD34(+) expanded cells were transplanted in sublethally irradiated NOD/SCID mice. With the three growth factor combination the CD34(+) cell number increased steadily up to the 8 weeks of culture. CD34(+) cells were expanded 67.5-fold with SF, 11.7 with HS and 49.2 with FCS. However, when CFCs and LTC-ICs were considered, a continuous expansion was observed only with HS and FCS, whereas in SF medium after 6 weeks their number started to decline. The addition of IL-6 did not change the expansion significantly. Cells grown ex vivo for 14 days were transplanted into NOD/SCID mice. The engraftment of human cells in mice was higher for serum-replete than for SF expanded cells. Nevertheless, SF cultured cells were also able to engraft both marrow and spleen in all animals. In addition, engrafted human cells still maintained clonogenic ability. With KL, FL, TPO +/- IL6 it is possible to expand haemopoietic progenitor cells in a SF medium. Compared with serum-replete cultures, the absolute number of clonogenic cells and in vivo repopulating cells is lower. Although the degree of expansion remains significant, a clinical trial still needs to be carried out to address the question of whether this expansion might be useful in reducing post-transplant aplasia." @default.
• W2322070115 created "2016-06-24" @default.
• W2322070115 creator A5000789355 @default.
• W2322070115 creator A5028372236 @default.
• W2322070115 creator A5034055285 @default.
• W2322070115 creator A5041442476 @default.
• W2322070115 creator A5041574361 @default.
• W2322070115 creator A5049670577 @default.
• W2322070115 creator A5056702454 @default.
• W2322070115 creator A5062571587 @default.
• W2322070115 creator A5062994642 @default.
• W2322070115 creator A5072883486 @default.
• W2322070115 creator A5073707704 @default.
• W2322070115 creator A5076059012 @default.
• W2322070115 date "2002-03-01" @default.
• W2322070115 modified "2023-10-07" @default.
• W2322070115 title "Role of different medium and growth factors on placental blood stem cell expansion: an in vitro and in vivo study" @default.
• W2322070115 cites W127246327 @default.
• W2322070115 cites W1532162953 @default.
• W2322070115 cites W1966100365 @default.
• W2322070115 cites W1976430696 @default.
• W2322070115 cites W1977253353 @default.
• W2322070115 cites W2003761566 @default.
• W2322070115 cites W2031979716 @default.
• W2322070115 cites W2049602391 @default.
• W2322070115 cites W2054031422 @default.
• W2322070115 cites W2076378257 @default.
• W2322070115 cites W2083466354 @default.
• W2322070115 cites W2084256703 @default.
• W2322070115 cites W2085516849 @default.
• W2322070115 cites W2116386021 @default.
• W2322070115 cites W2129321368 @default.
• W2322070115 cites W2134699909 @default.
• W2322070115 cites W2162833666 @default.
• W2322070115 cites W2272909078 @default.
• W2322070115 cites W2443124492 @default.
• W2322070115 doi "https://doi.org/10.1038/sj.bmt.1703390" @default.
• W2322070115 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11919735" @default.
• W2322070115 hasPublicationYear "2002" @default.
• W2322070115 type Work @default.
• W2322070115 sameAs 2322070115 @default.
• W2322070115 citedByCount "12" @default.
• W2322070115 countsByYear W23220701152017 @default.
• W2322070115 countsByYear W23220701152019 @default.
• W2322070115 crossrefType "journal-article" @default.
• W2322070115 hasAuthorship W2322070115A5000789355 @default.
• W2322070115 hasAuthorship W2322070115A5028372236 @default.
• W2322070115 hasAuthorship W2322070115A5034055285 @default.
• W2322070115 hasAuthorship W2322070115A5041442476 @default.
• W2322070115 hasAuthorship W2322070115A5041574361 @default.
• W2322070115 hasAuthorship W2322070115A5049670577 @default.
• W2322070115 hasAuthorship W2322070115A5056702454 @default.
• W2322070115 hasAuthorship W2322070115A5062571587 @default.
• W2322070115 hasAuthorship W2322070115A5062994642 @default.
• W2322070115 hasAuthorship W2322070115A5072883486 @default.
• W2322070115 hasAuthorship W2322070115A5073707704 @default.
• W2322070115 hasAuthorship W2322070115A5076059012 @default.
• W2322070115 hasBestOaLocation W23220701151 @default.
• W2322070115 hasConcept C10205521 @default.
• W2322070115 hasConcept C109159458 @default.
• W2322070115 hasConcept C126322002 @default.
• W2322070115 hasConcept C134018914 @default.
• W2322070115 hasConcept C150903083 @default.
• W2322070115 hasConcept C16685009 @default.
• W2322070115 hasConcept C201750760 @default.
• W2322070115 hasConcept C2022786 @default.
• W2322070115 hasConcept C203014093 @default.
• W2322070115 hasConcept C207001950 @default.
• W2322070115 hasConcept C26291073 @default.
• W2322070115 hasConcept C2777863708 @default.
• W2322070115 hasConcept C28328180 @default.
• W2322070115 hasConcept C71924100 @default.
• W2322070115 hasConcept C86803240 @default.
• W2322070115 hasConcept C95444343 @default.
• W2322070115 hasConceptScore W2322070115C10205521 @default.
• W2322070115 hasConceptScore W2322070115C109159458 @default.
• W2322070115 hasConceptScore W2322070115C126322002 @default.
• W2322070115 hasConceptScore W2322070115C134018914 @default.
• W2322070115 hasConceptScore W2322070115C150903083 @default.
• W2322070115 hasConceptScore W2322070115C16685009 @default.
• W2322070115 hasConceptScore W2322070115C201750760 @default.
• W2322070115 hasConceptScore W2322070115C2022786 @default.
• W2322070115 hasConceptScore W2322070115C203014093 @default.
• W2322070115 hasConceptScore W2322070115C207001950 @default.
• W2322070115 hasConceptScore W2322070115C26291073 @default.
• W2322070115 hasConceptScore W2322070115C2777863708 @default.
• W2322070115 hasConceptScore W2322070115C28328180 @default.
• W2322070115 hasConceptScore W2322070115C71924100 @default.
• W2322070115 hasConceptScore W2322070115C86803240 @default.
• W2322070115 hasConceptScore W2322070115C95444343 @default.
• W2322070115 hasIssue "5" @default.
• W2322070115 hasLocation W23220701151 @default.
• W2322070115 hasLocation W23220701152 @default.
• W2322070115 hasOpenAccess W2322070115 @default.
• W2322070115 hasPrimaryLocation W23220701151 @default.
• W2322070115 hasRelatedWork W1812483684 @default.
• W2322070115 hasRelatedWork W1998228373 @default.
• W2322070115 hasRelatedWork W2027401563 @default.

• next