FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2322236809> ?p ?o ?g. }

• W2322236809 endingPage "8" @default.
• W2322236809 startingPage "1" @default.
• W2322236809 abstract "Chytridiomycosis, caused by the fungal pathogen Batrachochytrium dendrobatidis (Bd), is responsible for massive declines and extinctions of amphibians worldwide. The most common method for detecting Bd is quantitative polymerase chain reaction (qPCR). qPCR is a highly sensitive detection technique, but its ability to determine the presence and accurately quantify the amount of Bd is also contingent on the efficiency of the DNA extraction method used prior to PCR. Using qPCR, we compared the extraction efficiency of 3 different extraction methods commonly used for Bd detection across a range of zoospore quantities: PrepMan Ultra Reagent, Qiagen DNeasy Blood and Tissue Kit, and Mobio PowerSoil DNA Isolation Kit. We show that not all extraction methods led to successful detection of Bd for the low zoospore quantities and that there was variation in the estimated zoospore equivalents among the methods, which demonstrates that these methods have different extraction efficiencies. These results highlight the importance of considering the extraction method when comparing across studies. The Qiagen DNeasy kit had the highest efficiency. We also show that replicated estimates of less than 1 zoospore can result from known zoospore concentrations; therefore, such results should be considered when obtained from field data. Additionally, we discuss the implications of our findings for interpreting previous studies and for conducting future Bd surveys. It is imperative to use the most efficient DNA extraction method in tandem with the highly sensitive qPCR technique in order to accurately diagnose the presence of Bd as well as other pathogens." @default.
• W2322236809 created "2016-06-24" @default.
• W2322236809 creator A5031467365 @default.
• W2322236809 creator A5034087494 @default.
• W2322236809 creator A5060892195 @default.
• W2322236809 date "2015-02-10" @default.
• W2322236809 modified "2023-10-09" @default.
• W2322236809 title "Differential efficiency among DNA extraction methods influences detection of the amphibian pathogen Batrachochytrium dendrobatidis" @default.
• W2322236809 cites W1924860219 @default.
• W2322236809 cites W1972017564 @default.
• W2322236809 cites W1986054653 @default.
• W2322236809 cites W1987557410 @default.
• W2322236809 cites W1989889539 @default.
• W2322236809 cites W1990480901 @default.
• W2322236809 cites W1998970048 @default.
• W2322236809 cites W2009184523 @default.
• W2322236809 cites W2009341256 @default.
• W2322236809 cites W2017888894 @default.
• W2322236809 cites W2023109738 @default.
• W2322236809 cites W2036241628 @default.
• W2322236809 cites W2039114788 @default.
• W2322236809 cites W2042586955 @default.
• W2322236809 cites W2049085830 @default.
• W2322236809 cites W2052368810 @default.
• W2322236809 cites W2056083982 @default.
• W2322236809 cites W2066597465 @default.
• W2322236809 cites W2070657242 @default.
• W2322236809 cites W2075179650 @default.
• W2322236809 cites W2081454596 @default.
• W2322236809 cites W2082907542 @default.
• W2322236809 cites W2087270985 @default.
• W2322236809 cites W2102956415 @default.
• W2322236809 cites W2105291817 @default.
• W2322236809 cites W2111760270 @default.
• W2322236809 cites W2121354575 @default.
• W2322236809 cites W2124422658 @default.
• W2322236809 cites W2130378728 @default.
• W2322236809 cites W2137037625 @default.
• W2322236809 cites W2139060243 @default.
• W2322236809 cites W2142756863 @default.
• W2322236809 cites W2143247233 @default.
• W2322236809 cites W2154337567 @default.
• W2322236809 doi "https://doi.org/10.3354/dao02822" @default.
• W2322236809 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25667331" @default.
• W2322236809 hasPublicationYear "2015" @default.
• W2322236809 type Work @default.
• W2322236809 sameAs 2322236809 @default.
• W2322236809 citedByCount "24" @default.
• W2322236809 countsByYear W23222368092015 @default.
• W2322236809 countsByYear W23222368092016 @default.
• W2322236809 countsByYear W23222368092017 @default.
• W2322236809 countsByYear W23222368092018 @default.
• W2322236809 countsByYear W23222368092019 @default.
• W2322236809 countsByYear W23222368092020 @default.
• W2322236809 countsByYear W23222368092021 @default.
• W2322236809 countsByYear W23222368092022 @default.
• W2322236809 crossrefType "journal-article" @default.
• W2322236809 hasAuthorship W2322236809A5031467365 @default.
• W2322236809 hasAuthorship W2322236809A5034087494 @default.
• W2322236809 hasAuthorship W2322236809A5060892195 @default.
• W2322236809 hasBestOaLocation W23222368091 @default.
• W2322236809 hasConcept C104317684 @default.
• W2322236809 hasConcept C113735248 @default.
• W2322236809 hasConcept C119128265 @default.
• W2322236809 hasConcept C174618031 @default.
• W2322236809 hasConcept C185592680 @default.
• W2322236809 hasConcept C18903297 @default.
• W2322236809 hasConcept C2776460866 @default.
• W2322236809 hasConcept C2781273459 @default.
• W2322236809 hasConcept C2781465898 @default.
• W2322236809 hasConcept C43617362 @default.
• W2322236809 hasConcept C4725764 @default.
• W2322236809 hasConcept C48023723 @default.
• W2322236809 hasConcept C49105822 @default.
• W2322236809 hasConcept C54355233 @default.
• W2322236809 hasConcept C552990157 @default.
• W2322236809 hasConcept C78063203 @default.
• W2322236809 hasConcept C86803240 @default.
• W2322236809 hasConcept C89423630 @default.
• W2322236809 hasConceptScore W2322236809C104317684 @default.
• W2322236809 hasConceptScore W2322236809C113735248 @default.
• W2322236809 hasConceptScore W2322236809C119128265 @default.
• W2322236809 hasConceptScore W2322236809C174618031 @default.
• W2322236809 hasConceptScore W2322236809C185592680 @default.
• W2322236809 hasConceptScore W2322236809C18903297 @default.
• W2322236809 hasConceptScore W2322236809C2776460866 @default.
• W2322236809 hasConceptScore W2322236809C2781273459 @default.
• W2322236809 hasConceptScore W2322236809C2781465898 @default.
• W2322236809 hasConceptScore W2322236809C43617362 @default.
• W2322236809 hasConceptScore W2322236809C4725764 @default.
• W2322236809 hasConceptScore W2322236809C48023723 @default.
• W2322236809 hasConceptScore W2322236809C49105822 @default.
• W2322236809 hasConceptScore W2322236809C54355233 @default.
• W2322236809 hasConceptScore W2322236809C552990157 @default.
• W2322236809 hasConceptScore W2322236809C78063203 @default.
• W2322236809 hasConceptScore W2322236809C86803240 @default.
• W2322236809 hasConceptScore W2322236809C89423630 @default.
• W2322236809 hasIssue "1" @default.

• next