Matches in SemOpenAlex for { <https://semopenalex.org/work/W2322249983> ?p ?o ?g. }
- W2322249983 endingPage "e1005966" @default.
- W2322249983 startingPage "e1005966" @default.
- W2322249983 abstract "Yra1 is an essential nuclear factor of the evolutionarily conserved family of hnRNP-like export factors that when overexpressed impairs mRNA export and cell growth. To investigate further the relevance of proper Yra1 stoichiometry in the cell, we overexpressed Yra1 by transforming yeast cells with YRA1 intron-less constructs and analyzed its effect on gene expression and genome integrity. We found that YRA1 overexpression induces DNA damage and leads to a transcription-associated hyperrecombination phenotype that is mediated by RNA:DNA hybrids. In addition, it confers a genome-wide replication retardation as seen by reduced BrdU incorporation and accumulation of the Rrm3 helicase. In addition, YRA1 overexpression causes a cell senescence-like phenotype and telomere shortening. ChIP-chip analysis shows that overexpressed Yra1 is loaded to transcribed chromatin along the genome and to Y' telomeric regions, where Rrm3 is also accumulated, suggesting an impairment of telomere replication. Our work not only demonstrates that a proper stoichiometry of the Yra1 mRNA binding and export factor is required to maintain genome integrity and telomere homeostasis, but suggests that the cellular imbalance between transcribed RNA and specific RNA-binding factors may become a major cause of genome instability mediated by co-transcriptional replication impairment." @default.
- W2322249983 created "2016-06-24" @default.
- W2322249983 creator A5036367000 @default.
- W2322249983 creator A5043281613 @default.
- W2322249983 creator A5054797076 @default.
- W2322249983 creator A5063444330 @default.
- W2322249983 creator A5067249637 @default.
- W2322249983 date "2016-04-01" @default.
- W2322249983 modified "2023-10-02" @default.
- W2322249983 title "Excess of Yra1 RNA-Binding Factor Causes Transcription-Dependent Genome Instability, Replication Impairment and Telomere Shortening" @default.
- W2322249983 cites W1007570554 @default.
- W2322249983 cites W14462737 @default.
- W2322249983 cites W1502616762 @default.
- W2322249983 cites W1524334293 @default.
- W2322249983 cites W1557484961 @default.
- W2322249983 cites W1651648648 @default.
- W2322249983 cites W1923529605 @default.
- W2322249983 cites W1966017154 @default.
- W2322249983 cites W1966735041 @default.
- W2322249983 cites W1968593031 @default.
- W2322249983 cites W1968789240 @default.
- W2322249983 cites W1970240865 @default.
- W2322249983 cites W1971776319 @default.
- W2322249983 cites W1972011626 @default.
- W2322249983 cites W1975593054 @default.
- W2322249983 cites W1976019768 @default.
- W2322249983 cites W1977343831 @default.
- W2322249983 cites W1977646116 @default.
- W2322249983 cites W1977851916 @default.
- W2322249983 cites W1984352890 @default.
- W2322249983 cites W1985057553 @default.
- W2322249983 cites W1991002538 @default.
- W2322249983 cites W1993657453 @default.
- W2322249983 cites W1995842607 @default.
- W2322249983 cites W1997493439 @default.
- W2322249983 cites W2000456081 @default.
- W2322249983 cites W2003025826 @default.
- W2322249983 cites W2012466702 @default.
- W2322249983 cites W2017629702 @default.
- W2322249983 cites W2018106885 @default.
- W2322249983 cites W2019246380 @default.
- W2322249983 cites W2026686272 @default.
- W2322249983 cites W2027106670 @default.
- W2322249983 cites W2030978639 @default.
- W2322249983 cites W2031658439 @default.
- W2322249983 cites W2032742580 @default.
- W2322249983 cites W2033054103 @default.
- W2322249983 cites W2035300822 @default.
- W2322249983 cites W2036232815 @default.
- W2322249983 cites W2038749586 @default.
- W2322249983 cites W2040410152 @default.
- W2322249983 cites W2042409563 @default.
- W2322249983 cites W2042969047 @default.
- W2322249983 cites W2043375872 @default.
- W2322249983 cites W2045529026 @default.
- W2322249983 cites W2047507363 @default.
- W2322249983 cites W2049314528 @default.
- W2322249983 cites W2058108472 @default.
- W2322249983 cites W2059453176 @default.
- W2322249983 cites W2065677302 @default.
- W2322249983 cites W2070380559 @default.
- W2322249983 cites W2075571819 @default.
- W2322249983 cites W2076091831 @default.
- W2322249983 cites W2076367887 @default.
- W2322249983 cites W2077323302 @default.
- W2322249983 cites W2077430509 @default.
- W2322249983 cites W2078403259 @default.
- W2322249983 cites W2080862728 @default.
- W2322249983 cites W2087576311 @default.
- W2322249983 cites W2089890829 @default.
- W2322249983 cites W2094569502 @default.
- W2322249983 cites W2094931306 @default.
- W2322249983 cites W2095881318 @default.
- W2322249983 cites W2101359917 @default.
- W2322249983 cites W2103694115 @default.
- W2322249983 cites W2107712228 @default.
- W2322249983 cites W2109052166 @default.
- W2322249983 cites W2109354723 @default.
- W2322249983 cites W2109845157 @default.
- W2322249983 cites W2112348939 @default.
- W2322249983 cites W2112500667 @default.
- W2322249983 cites W2116114189 @default.
- W2322249983 cites W2119160686 @default.
- W2322249983 cites W2120143583 @default.
- W2322249983 cites W2124242199 @default.
- W2322249983 cites W2125433175 @default.
- W2322249983 cites W2126525177 @default.
- W2322249983 cites W2127129859 @default.
- W2322249983 cites W2127598257 @default.
- W2322249983 cites W2129214044 @default.
- W2322249983 cites W2130443336 @default.
- W2322249983 cites W2137990405 @default.
- W2322249983 cites W2139028412 @default.
- W2322249983 cites W2139879864 @default.
- W2322249983 cites W2142125232 @default.
- W2322249983 cites W2155915917 @default.
- W2322249983 cites W2156315323 @default.
- W2322249983 cites W2163765191 @default.