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• W2322276615 abstract "Tissue cyst formation by a goat isolate (GT-1) of Toxoplasma gondii was examined in bovine monocyte, human fetal lung, and Madin-Darby bovine kidney cell cultures. Transmission electron microscopy (TEM) and cat feeding studies indicated that tissue cysts were present in all 3 cell lines examined. Tissue cysts were first seen 3 days postinoculation (PI) using TEM. Standard cell culture procedures were used and no additional condition was needed to induce tissue cyst formation. Cats fed cell cultures excreted T. gondii oocysts in their feces 5-7 days PI. These oocysts caused lethal infections in mice. Tissue cysts were produced in cell cultures regardless if the initiating inoculum consisted of bradyzoites, sporozoites, or a mixture of bradyzoites and tachyzoites. Tissue cyst formation has been followed through 40 subpassages of infected cells. By TEM tissue cysts still were present after 40 passages, but when 40th-passaged cultures were fed to cats, oocytsts were not excreted. This indicates that the parasite had become oocystless after repeated passage in vitro." @default.
• W2322276615 created "2016-06-24" @default.
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• W2322276615 date "1991-02-01" @default.
• W2322276615 modified "2023-10-16" @default.
• W2322276615 title "Examination of Tissue Cyst Formation by Toxoplasma gondii in Cell Cultures Using Bradyzoites, Tachyzoites, and Sporozoites" @default.
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• W2322276615 doi "https://doi.org/10.2307/3282569" @default.
• W2322276615 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1992083" @default.
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