FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2322369524> ?p ?o ?g. }

• W2322369524 abstract "Lampreys are extant representatives of agnathans. Descriptions of lamprey development, physiology and genome have provided critical insights into early evolution of vertebrate traits. However, efficient means for genetic manipulation in agnathan species have not been developed, hindering functional studies of genes in these important Evo-Devo models. Here, we report a CRISPR/Cas system optimized for lamprey genomes and use it to disrupt genomic loci in the Northeast Chinese lamprey (Lethenteron morii) with efficiencies ranging between 84~99%. The frequencies of indels observed in the target loci of golden (gol), kctd10, wee1, soxe2, and wnt7b, estimated from direct sequencing of genomic DNA samples of injected lamprey larvae, were 68/69, 47/56, 38/39, 36/37 and 36/42, respectively. These indels often occurred in both alleles. In the CRISPR/Cas9 treatment for gol or kctd10, 38.6% or 85.3% of the targeted larvae had the respective recessive null-like phenotypes, further confirming the disruption of both loci. The kctd10 gRNA, designed against an essential functional region of Kctd10, resulted in null-like phenotypes and in-frame mutations in alleles. We suggest that the CRISPR/Cas-based approach has the potential for efficient genetic perturbation in organisms less amenable to germ line transmission based approaches." @default.
• W2322369524 created "2016-06-24" @default.
• W2322369524 creator A5005784160 @default.
• W2322369524 creator A5006882891 @default.
• W2322369524 creator A5046621948 @default.
• W2322369524 creator A5065197431 @default.
• W2322369524 creator A5087562556 @default.
• W2322369524 creator A5087855509 @default.
• W2322369524 creator A5090092746 @default.
• W2322369524 creator A5091610752 @default.
• W2322369524 date "2016-03-01" @default.
• W2322369524 modified "2023-10-12" @default.
• W2322369524 title "Biallelic editing of a lamprey genome using the CRISPR/Cas9 system" @default.
• W2322369524 cites W1128116721 @default.
• W2322369524 cites W1850661934 @default.
• W2322369524 cites W1932232911 @default.
• W2322369524 cites W1963843776 @default.
• W2322369524 cites W1967825495 @default.
• W2322369524 cites W1976391205 @default.
• W2322369524 cites W2001497035 @default.
• W2322369524 cites W2004320797 @default.
• W2322369524 cites W2009371644 @default.
• W2322369524 cites W2016405544 @default.
• W2322369524 cites W2017224987 @default.
• W2322369524 cites W2020448266 @default.
• W2322369524 cites W2029985695 @default.
• W2322369524 cites W2038659500 @default.
• W2322369524 cites W2041211823 @default.
• W2322369524 cites W2043474702 @default.
• W2322369524 cites W2052131772 @default.
• W2322369524 cites W2060632930 @default.
• W2322369524 cites W2063807032 @default.
• W2322369524 cites W2067555693 @default.
• W2322369524 cites W2069584416 @default.
• W2322369524 cites W2075180196 @default.
• W2322369524 cites W2081928322 @default.
• W2322369524 cites W2083861837 @default.
• W2322369524 cites W2085894715 @default.
• W2322369524 cites W2087668227 @default.
• W2322369524 cites W2090015201 @default.
• W2322369524 cites W2091442434 @default.
• W2322369524 cites W2092806325 @default.
• W2322369524 cites W2099326239 @default.
• W2322369524 cites W2112444085 @default.
• W2322369524 cites W2119771879 @default.
• W2322369524 cites W2122375374 @default.
• W2322369524 cites W2124668406 @default.
• W2322369524 cites W2125349610 @default.
• W2322369524 cites W2133702185 @default.
• W2322369524 cites W2136963255 @default.
• W2322369524 cites W2137209165 @default.
• W2322369524 cites W2137233414 @default.
• W2322369524 cites W2162096419 @default.
• W2322369524 cites W2170039745 @default.
• W2322369524 cites W4240053492 @default.
• W2322369524 doi "https://doi.org/10.1038/srep23496" @default.
• W2322369524 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4804306" @default.
• W2322369524 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27005311" @default.
• W2322369524 hasPublicationYear "2016" @default.
• W2322369524 type Work @default.
• W2322369524 sameAs 2322369524 @default.
• W2322369524 citedByCount "46" @default.
• W2322369524 countsByYear W23223695242017 @default.
• W2322369524 countsByYear W23223695242018 @default.
• W2322369524 countsByYear W23223695242019 @default.
• W2322369524 countsByYear W23223695242020 @default.
• W2322369524 countsByYear W23223695242021 @default.
• W2322369524 countsByYear W23223695242022 @default.
• W2322369524 countsByYear W23223695242023 @default.
• W2322369524 crossrefType "journal-article" @default.
• W2322369524 hasAuthorship W2322369524A5005784160 @default.
• W2322369524 hasAuthorship W2322369524A5006882891 @default.
• W2322369524 hasAuthorship W2322369524A5046621948 @default.
• W2322369524 hasAuthorship W2322369524A5065197431 @default.
• W2322369524 hasAuthorship W2322369524A5087562556 @default.
• W2322369524 hasAuthorship W2322369524A5087855509 @default.
• W2322369524 hasAuthorship W2322369524A5090092746 @default.
• W2322369524 hasAuthorship W2322369524A5091610752 @default.
• W2322369524 hasBestOaLocation W23223695241 @default.
• W2322369524 hasConcept C104317684 @default.
• W2322369524 hasConcept C119054055 @default.
• W2322369524 hasConcept C127716648 @default.
• W2322369524 hasConcept C132455925 @default.
• W2322369524 hasConcept C135763542 @default.
• W2322369524 hasConcept C141231307 @default.
• W2322369524 hasConcept C144501496 @default.
• W2322369524 hasConcept C153209595 @default.
• W2322369524 hasConcept C180754005 @default.
• W2322369524 hasConcept C19843653 @default.
• W2322369524 hasConcept C2780417240 @default.
• W2322369524 hasConcept C2910714999 @default.
• W2322369524 hasConcept C505870484 @default.
• W2322369524 hasConcept C54355233 @default.
• W2322369524 hasConcept C70721500 @default.
• W2322369524 hasConcept C78458016 @default.
• W2322369524 hasConcept C86803240 @default.
• W2322369524 hasConcept C98108389 @default.
• W2322369524 hasConceptScore W2322369524C104317684 @default.
• W2322369524 hasConceptScore W2322369524C119054055 @default.
• W2322369524 hasConceptScore W2322369524C127716648 @default.

• next