Matches in SemOpenAlex for { <https://semopenalex.org/work/W2322372110> ?p ?o ?g. }
- W2322372110 endingPage "2621" @default.
- W2322372110 startingPage "2613" @default.
- W2322372110 abstract "The canonical heme oxygenases (HOs) catalyze heme oxidation via a heme-bound hydroperoxo intermediate that is stabilized by a water cluster at the active site of the enzyme. In contrast, the hydrophobic active site of IsdI, a heme-degrading enzyme from Staphylococcus aureus, lacks a water cluster and is expected to oxidize heme by an alternative mechanism. Reaction of the IsdI–heme complex with either H2O2 or m-chloroperoxybenzoic acid fails to produce a specific oxidized heme iron intermediate, suggesting that ferric–hydroperoxo or ferryl derivatives of IsdI are not involved in the catalytic mechanism of this enzyme. IsdI lacks a proton-donating group in the distal heme pocket, so the possible involvement of a ferric–peroxo intermediate has been evaluated. Density functional theory (DFT) calculations indicate that heme oxidation involving a ferric–peroxo intermediate is energetically accessible, whereas the energy barrier for a reaction involving a ferric–hydroperoxo intermediate is too great in the absence of a proton donor. We propose that IsdI catalyzes heme oxidation through nucleophilic attack by the heme-bound peroxo species. This proposal is consistent with our previous demonstration by nuclear magnetic resonance spectroscopy that heme ruffling increases the susceptibility of the meso-carbon of heme to nucleophilic attack." @default.
- W2322372110 created "2016-06-24" @default.
- W2322372110 creator A5053944639 @default.
- W2322372110 creator A5071277571 @default.
- W2322372110 creator A5083877410 @default.
- W2322372110 creator A5091229812 @default.
- W2322372110 date "2015-04-16" @default.
- W2322372110 modified "2023-09-27" @default.
- W2322372110 title "A Ferric–Peroxo Intermediate in the Oxidation of Heme by IsdI" @default.
- W2322372110 cites W1490103358 @default.
- W2322372110 cites W1572334445 @default.
- W2322372110 cites W1586487499 @default.
- W2322372110 cites W1929067551 @default.
- W2322372110 cites W1967403912 @default.
- W2322372110 cites W1968547522 @default.
- W2322372110 cites W1970675158 @default.
- W2322372110 cites W1971624564 @default.
- W2322372110 cites W1975262546 @default.
- W2322372110 cites W1979363917 @default.
- W2322372110 cites W1980001065 @default.
- W2322372110 cites W1981253403 @default.
- W2322372110 cites W1983392880 @default.
- W2322372110 cites W1989585425 @default.
- W2322372110 cites W1991610652 @default.
- W2322372110 cites W2001406413 @default.
- W2322372110 cites W2003482250 @default.
- W2322372110 cites W2006840128 @default.
- W2322372110 cites W2018578574 @default.
- W2322372110 cites W2021090633 @default.
- W2322372110 cites W2022482348 @default.
- W2322372110 cites W2023558879 @default.
- W2322372110 cites W2025766479 @default.
- W2322372110 cites W2029181830 @default.
- W2322372110 cites W2032090992 @default.
- W2322372110 cites W2034329041 @default.
- W2322372110 cites W2038554626 @default.
- W2322372110 cites W2042183419 @default.
- W2322372110 cites W2043583102 @default.
- W2322372110 cites W2053530773 @default.
- W2322372110 cites W2062583944 @default.
- W2322372110 cites W2063140678 @default.
- W2322372110 cites W2063677803 @default.
- W2322372110 cites W2066007975 @default.
- W2322372110 cites W2068995800 @default.
- W2322372110 cites W2072206903 @default.
- W2322372110 cites W2073790538 @default.
- W2322372110 cites W2073932230 @default.
- W2322372110 cites W2074218075 @default.
- W2322372110 cites W2075381847 @default.
- W2322372110 cites W2076555138 @default.
- W2322372110 cites W2083630923 @default.
- W2322372110 cites W2086209070 @default.
- W2322372110 cites W2086675509 @default.
- W2322372110 cites W2101897226 @default.
- W2322372110 cites W2119635659 @default.
- W2322372110 cites W2124064813 @default.
- W2322372110 cites W2143226173 @default.
- W2322372110 cites W2144674829 @default.
- W2322372110 cites W2150300764 @default.
- W2322372110 cites W2154063105 @default.
- W2322372110 cites W2168427863 @default.
- W2322372110 doi "https://doi.org/10.1021/acs.biochem.5b00239" @default.
- W2322372110 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25853501" @default.
- W2322372110 hasPublicationYear "2015" @default.
- W2322372110 type Work @default.
- W2322372110 sameAs 2322372110 @default.
- W2322372110 citedByCount "14" @default.
- W2322372110 countsByYear W23223721102016 @default.
- W2322372110 countsByYear W23223721102017 @default.
- W2322372110 countsByYear W23223721102018 @default.
- W2322372110 countsByYear W23223721102019 @default.
- W2322372110 countsByYear W23223721102020 @default.
- W2322372110 countsByYear W23223721102022 @default.
- W2322372110 countsByYear W23223721102023 @default.
- W2322372110 crossrefType "journal-article" @default.
- W2322372110 hasAuthorship W2322372110A5053944639 @default.
- W2322372110 hasAuthorship W2322372110A5071277571 @default.
- W2322372110 hasAuthorship W2322372110A5083877410 @default.
- W2322372110 hasAuthorship W2322372110A5091229812 @default.
- W2322372110 hasConcept C161790260 @default.
- W2322372110 hasConcept C178790620 @default.
- W2322372110 hasConcept C178907741 @default.
- W2322372110 hasConcept C179104552 @default.
- W2322372110 hasConcept C181199279 @default.
- W2322372110 hasConcept C185592680 @default.
- W2322372110 hasConcept C2776217839 @default.
- W2322372110 hasConcept C2780534640 @default.
- W2322372110 hasConcept C71240020 @default.
- W2322372110 hasConcept C75473681 @default.
- W2322372110 hasConceptScore W2322372110C161790260 @default.
- W2322372110 hasConceptScore W2322372110C178790620 @default.
- W2322372110 hasConceptScore W2322372110C178907741 @default.
- W2322372110 hasConceptScore W2322372110C179104552 @default.
- W2322372110 hasConceptScore W2322372110C181199279 @default.
- W2322372110 hasConceptScore W2322372110C185592680 @default.
- W2322372110 hasConceptScore W2322372110C2776217839 @default.
- W2322372110 hasConceptScore W2322372110C2780534640 @default.
- W2322372110 hasConceptScore W2322372110C71240020 @default.