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- W2322420739 abstract "Introduction: Accurate quantification of mycobacterial load is important for the evaluation of patient infectiousness, disease severity and monitoring treatment response in human and in-vitro laboratory models of disease. We hypothesized that newer techniques would perform as well as solid media culture to quantify mycobacterial burden in laboratory specimens. Methods: We compared the turn-around-time, detection-threshold, dynamic range, reproducibility, relative discriminative ability, of 4 mycobacterial load determination techniques: automated liquid culture (BACTEC-MGIT-960), [ 3 H]-uracil incorporation assays, luciferase-reporter construct bioluminescence, and quantitative PCR(Xpert -MTB/RIF) using serial dilutions of Mycobacterium bovis and Mycobacterium tuberculosis H37RV. Mycobacterial colony-forming-units(CFU) using 7H10-Middlebrook solid media served as the reference standard. Results: All 4 assays correlated well with the reference standard, however, bioluminescence and uracil assays had a" @default.
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- W2322420739 date "2012-05-01" @default.
- W2322420739 modified "2023-09-25" @default.
- W2322420739 title "Comparison Of Quantitative Techniques Including Xpert MTB/RIF To Evaluate Mycobacterial Burden" @default.
- W2322420739 doi "https://doi.org/10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a4714" @default.
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