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• W2322888916 abstract "Event Abstract Back to Event 3D bioprinting of multi-biomaterial/crosslinked bioink for skin tissue engineering Hongbo Zhang1, Ke Yang1, Gongcheng Liu2, Shihui Zhu2, Ruixue Yin1 and Wenjun Zhang3 1 East China University of Science and Technology, Complex and Intelligent Research Centre, China 2 Changhai hospital, China 3 University of Saskatchewan, Biomedical engineering, Canada In this paper, multi-biomaterials with a novel crosslinking process (combination of pre-chemical and physical crosslinking method ) were used to form a bioink to produce 3D cell-laden constructs by 3D bioprinting for skin tissue engineering. The skin constructs produced by the bioink showed an improved mechanical strength that matched the human skin compared with the bioink by physical crosslinked only. Prolonged degradation rate was also achieved. The bioink was also biocompatible, high cell surviving rate was achieved. Introduction: 3D bioprinting has shown a great promise in fabricating 3D complex multicellular tissues analog living architecture by delivering a precisely cell population encapsulated by bioinks to a targeted location. However, the limited choice of 3D-printable bioinks for cell encapsulation and supporting scaffolds is the main obstacle for 3D bioprinting[1],[2]. In this paper, a composition of Collagen, Hyaluronic Acid (HA) and Chitosan was chosen to develop a biocompatible bioink. Two crosslinking methods, named pre-chemical crosslinking before printing and physical crosslinking after printing, were applied to achieve desired mechanical strength and biocompatibility. Fibroblasts (FBs) were dispersed into the bioink to produced 3D cell-laden living constructs. Materials and Methods: ChitosanHACollagen mixtures were blended with different weight ratios. 3 wt%, 6 wt% and 9 wt% of Tyrosinase in deionized water were added to the mixtures before printing to test the pre-chemical crosslinking process, the samples were denoted as ChsHACol -T, samples without Tyrosinase pre-chemical crosslinking were denoted as ChsHACol. 3D constructs of ChsHACol and ChsHACol-T scaffolds were fabricated by a 3D bioplotter (EnvisionTEC, Germany). Sterilized sodium tripolyphosphate (STPP) solution (15 wt%) was nebulized to physically crosslink the hydrogels for 15min for each layer. Results: The composition of the bioink of chitosan of 8 wt%, HA of 0.5 wt%, and 10wt% of collagen was chosen to produce skin constructs. The morphology of the constructs resembled human skin and had a layered structure as shown in Figure 1. The tensile strength of ChsHACol and ChsHACol-T were 29.9 and 39.1kPa, respectively. The Young’s modulus of ChsHACol and ChsHACol-T were 0.170 and 0.313MPa. These results indicated that ChsHACol-T offered an improved mechanical strength that matched the human skin[3] due to chemical-induced cross-linking by tyrosinase. Figure 1 The degradation rates of ChsHACol and ChsHACol-T in 35 days were 81.99% and 55.34%, respectively. It indicated that using tyrosinase pre-chemical crosslinked hydrogel could slow down the degradation of the skin constructs as shown in Figure 2a, b. The cell-culture showed that the skin constructs are biocompatible and have a high cell surviving rate as shown in Figure 2c. Figure 2 Conclusions: A novel bioink for 3D bioprinting was obtained by a pre-chemical crosslinking method combined with physical crosslinking of multi-biomaterials to produce skin substitutes. The results showed that the skin constructs by this novel crosslinking process exhibited good mechanical properties and biocompatibility. The mechanical strength and degradation rate can be tailed by the concentration of tyrosinase. Thus, this novel cross-linking method is promising is soft tissue engineering, such as skin, cartilage tissue engineering. References:[1] A. L. Rutz , K.E. Hyland , A. E. Jakus , W. R. Burghardt , and Ramille N. Shah, A Multimaterial Bioink Method for 3D Printing Tunable, Cell-Compatible Hydrogels, Advanced Materials, 2015, 27, 1607–1614.[2] F. M. Wood, Skin regeneration: The complexities of translation into clinical practice, The International Journal of Biochemistry & Cell Biology 56, 2014, 133–140.[3] F. H.Zulkifli, F. S Hussain,M. S. Rasad, M. M. Yusoff , Nanostructured materials from hydroxyethyl cellulose for skintissue engineering, Carbohydrate Polymers 114 (2014) 238–245. Keywords: gel, Bioprinting, 3D scaffold, Burn wound Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: New Frontier Oral Topic: Biomaterials in constructing tissue substitutes Citation: Zhang H, Yang K, Liu G, Zhu S, Yin R and Zhang W (2016). 3D bioprinting of multi-biomaterial/crosslinked bioink for skin tissue engineering. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02354 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Hongbo Zhang Ke Yang Gongcheng Liu Shihui Zhu Ruixue Yin Wenjun Zhang Google Hongbo Zhang Ke Yang Gongcheng Liu Shihui Zhu Ruixue Yin Wenjun Zhang Google Scholar Hongbo Zhang Ke Yang Gongcheng Liu Shihui Zhu Ruixue Yin Wenjun Zhang PubMed Hongbo Zhang Ke Yang Gongcheng Liu Shihui Zhu Ruixue Yin Wenjun Zhang Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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• W2322888916 title "3D bioprinting of multi-biomaterial/crosslinked bioink for skin tissue engineering" @default.
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