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- W2322983886 abstract "Purpose: Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained great interest as a promising cell source for regenerative medicine due to non-invasive collection, readily availability, high expansion capacity, and low immunogenicity. We investigated the feasibility of transplanting hUCB-MSCs and a hyaluronic acid (HA) hydrogel composite to repair full-thickness articular cartilage defects and determine whether the transplanted cells disappeared from the defect site. Methods: Full-thickness osteochondral defects (3 mm in diameter and 3 mm depth) were created in the trochlear groove of the femur. A composite of hUCB-MSCs (0.5 × 107 cells/mL) and 4% HA hydrogel was transplanted into the full-thickness defect in the experimental knee (right knee), whereas 4% HA hydrogel without hUCB-MSCs was transplanted into the control knee (left knee). Animals were sacrificed at 8 and 16 weeks post-transplantation. The articular cartilage defect repair was evaluated grossly and histologically. Histological images were assessed with the modified O’Driscoll score. The mouse anti-human nuclei monoclonal antibody was applied to tissues for cell tracking analysis. Results: Transplanting hUCB-MSCs and a HA hydrogel composite resulted in overall superior cartilage repair tissue with better quality than HA alone or no treatment. Cellular architecture and collagen arrangement at 16 weeks were similar to those of surrounding normal articular cartilage tissue. The histological scores also revealed that cartilage repair in experimental knees were better compared to control knees. The grafted cells were tracked by immunohistochemistry using anti-human nuclei antibody after transplanting the cells. Anti-human nuclei antibody staining revealed gradual decrease of the transplanted cells at the region of the cartilage defect in 2, 4, and 8 weeks post-transplantation. Conclusions: This study demonstrated that hUCB-MSCs and a 4% HA hydrogel composite resulted in favorable cartilage repair grossly and histologically compared to HA only and defect only in a rabbit model. The transplanted cells disappeared in the repair tissue over time, which supported the paracrine action of transplanted MSCS for cartilage repair rather than direct differentiation. These findings suggest that transplanting hUCB-MSCs and a HA hydrogel composite can be a novel therapeutic modality to treat full-thickness cartilage defects." @default.
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- W2322983886 date "2016-04-01" @default.
- W2322983886 modified "2023-10-14" @default.
- W2322983886 title "Human umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel composite promotes the articular cartilage repair in a rabbit model" @default.
- W2322983886 doi "https://doi.org/10.1016/j.joca.2016.01.844" @default.
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