Matches in SemOpenAlex for { <https://semopenalex.org/work/W2323348506> ?p ?o ?g. }
- W2323348506 endingPage "C919" @default.
- W2323348506 startingPage "C910" @default.
- W2323348506 abstract "Stiffness of live cardiomyocytes isolated from control and diabetic mice was measured using the atomic force microscopy nanoindentation method. Type 1 diabetes was induced in mice by streptozotocin administration. Histological images of myocardium from mice that were diabetic for 3 mo showed disorderly lineup of myocardial cells, irregularly sized cell nuclei, and fragmented and disordered myocardial fibers with interstitial collagen accumulation. Phalloidin-stained cardiomyocytes isolated from diabetic mice showed altered (i.e., more irregular and diffuse) actin filament organization compared with cardiomyocytes from control mice. Sarco/endoplasmic reticulum Ca 2+ -ATPase (SERCA2a) pump expression was reduced in homogenates obtained from the left ventricle of diabetic animals compared with age-matched controls. The apparent elastic modulus (AEM) for live control or diabetic isolated cardiomyocytes was measured using the atomic force microscopy nanoindentation method in Tyrode buffer solution containing 1.8 mM Ca 2+ and 5.4 mM KCl (physiological condition), 100 nM Ca 2+ and 5.4 mM KCl (low extracellular Ca 2+ condition), or 1.8 mM Ca 2+ and 140 mM KCl (contraction condition). In the physiological condition, the mean AEM was 112% higher for live diabetic than control isolated cardiomyocytes (91 ± 14 vs. 43 ± 7 kPa). The AEM was also significantly higher in diabetic than control cardiomyocytes in the low extracellular Ca 2+ and contraction conditions. These findings suggest that the material properties of live cardiomyocytes were affected by diabetes, resulting in stiffer cells, which very likely contribute to high diastolic LV stiffness, which has been observed in vivo in some diabetes mellitus patients." @default.
- W2323348506 created "2016-06-24" @default.
- W2323348506 creator A5010413199 @default.
- W2323348506 creator A5043533469 @default.
- W2323348506 creator A5052637416 @default.
- W2323348506 creator A5053087366 @default.
- W2323348506 creator A5059759367 @default.
- W2323348506 creator A5067489504 @default.
- W2323348506 creator A5082900919 @default.
- W2323348506 date "2014-11-15" @default.
- W2323348506 modified "2023-09-25" @default.
- W2323348506 title "Diabetes increases stiffness of live cardiomyocytes measured by atomic force microscopy nanoindentation" @default.
- W2323348506 cites W1626890019 @default.
- W2323348506 cites W1963915666 @default.
- W2323348506 cites W1971030181 @default.
- W2323348506 cites W1977325118 @default.
- W2323348506 cites W1982079739 @default.
- W2323348506 cites W1986093704 @default.
- W2323348506 cites W1991520769 @default.
- W2323348506 cites W1999970092 @default.
- W2323348506 cites W2000755132 @default.
- W2323348506 cites W2008289905 @default.
- W2323348506 cites W2014051267 @default.
- W2323348506 cites W2020267609 @default.
- W2323348506 cites W2030642623 @default.
- W2323348506 cites W2040603772 @default.
- W2323348506 cites W2042010963 @default.
- W2323348506 cites W2051131221 @default.
- W2323348506 cites W2059963275 @default.
- W2323348506 cites W2060431351 @default.
- W2323348506 cites W2067272053 @default.
- W2323348506 cites W2070647639 @default.
- W2323348506 cites W2071555363 @default.
- W2323348506 cites W2082295516 @default.
- W2323348506 cites W2086622196 @default.
- W2323348506 cites W2087466511 @default.
- W2323348506 cites W2099356473 @default.
- W2323348506 cites W2101045288 @default.
- W2323348506 cites W2102297386 @default.
- W2323348506 cites W2122098350 @default.
- W2323348506 cites W2126284874 @default.
- W2323348506 cites W2128269140 @default.
- W2323348506 cites W2128300830 @default.
- W2323348506 cites W2129241979 @default.
- W2323348506 cites W2140058582 @default.
- W2323348506 cites W2153900353 @default.
- W2323348506 cites W2168624052 @default.
- W2323348506 cites W2262793924 @default.
- W2323348506 cites W2461980944 @default.
- W2323348506 cites W4252437054 @default.
- W2323348506 doi "https://doi.org/10.1152/ajpcell.00192.2013" @default.
- W2323348506 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25163520" @default.
- W2323348506 hasPublicationYear "2014" @default.
- W2323348506 type Work @default.
- W2323348506 sameAs 2323348506 @default.
- W2323348506 citedByCount "38" @default.
- W2323348506 countsByYear W23233485062014 @default.
- W2323348506 countsByYear W23233485062015 @default.
- W2323348506 countsByYear W23233485062016 @default.
- W2323348506 countsByYear W23233485062017 @default.
- W2323348506 countsByYear W23233485062018 @default.
- W2323348506 countsByYear W23233485062019 @default.
- W2323348506 countsByYear W23233485062020 @default.
- W2323348506 countsByYear W23233485062021 @default.
- W2323348506 countsByYear W23233485062022 @default.
- W2323348506 countsByYear W23233485062023 @default.
- W2323348506 crossrefType "journal-article" @default.
- W2323348506 hasAuthorship W2323348506A5010413199 @default.
- W2323348506 hasAuthorship W2323348506A5043533469 @default.
- W2323348506 hasAuthorship W2323348506A5052637416 @default.
- W2323348506 hasAuthorship W2323348506A5053087366 @default.
- W2323348506 hasAuthorship W2323348506A5059759367 @default.
- W2323348506 hasAuthorship W2323348506A5067489504 @default.
- W2323348506 hasAuthorship W2323348506A5082900919 @default.
- W2323348506 hasConcept C102951782 @default.
- W2323348506 hasConcept C12554922 @default.
- W2323348506 hasConcept C125705527 @default.
- W2323348506 hasConcept C126322002 @default.
- W2323348506 hasConcept C134018914 @default.
- W2323348506 hasConcept C158617107 @default.
- W2323348506 hasConcept C159985019 @default.
- W2323348506 hasConcept C163415756 @default.
- W2323348506 hasConcept C171250308 @default.
- W2323348506 hasConcept C174054327 @default.
- W2323348506 hasConcept C185592680 @default.
- W2323348506 hasConcept C189165786 @default.
- W2323348506 hasConcept C192562407 @default.
- W2323348506 hasConcept C207200792 @default.
- W2323348506 hasConcept C2776553924 @default.
- W2323348506 hasConcept C2778198053 @default.
- W2323348506 hasConcept C2778797674 @default.
- W2323348506 hasConcept C2778921608 @default.
- W2323348506 hasConcept C2779280383 @default.
- W2323348506 hasConcept C28406088 @default.
- W2323348506 hasConcept C49326732 @default.
- W2323348506 hasConcept C55493867 @default.
- W2323348506 hasConcept C555293320 @default.
- W2323348506 hasConcept C68731436 @default.