Matches in SemOpenAlex for { <https://semopenalex.org/work/W2323429265> ?p ?o ?g. }
Showing items 1 to 59 of
59
with 100 items per page.
- W2323429265 endingPage "1911" @default.
- W2323429265 startingPage "1905" @default.
- W2323429265 abstract "The IMP-degrading enzyme from ordinary muscle of the snapper Pagrus auratus was purified by ammonium sulfate fractionation and two steps of affinity chromatography on Con A-Sepharose and 5'-AMP Sepharose 4B. The purified enzyme was shown to be homogeneous by disc polyacrylamide gel electrophoresis. The apparent molecular weight of the purified enzyme was estimated to be about 8.9×104 by SDS-polyacrylamide gel electrophoresis and about 3.6×105 by gel filtration with Sephacryl S-300HR, thus this enzyme was shown to be a tetramer. The purified enzyme was identified as 5'-nucleotidase (EC 3.1.3.5) by substrate specificity, it has maximal activity at pH 8.5 in Tris-acetate buffer in the presence of MgC12, and it hydrolyzed 5'-AMP most effectively. Of several metal ions, Mn2+ was the most effective activator and Co2+ also activated this enzyme. The enzyme activity was inhibited by Zn2+, Cu2+, Bat2+, and EDTA and complete inhibition of the enzyme occurred in the presence of 1 mM 5'-ATP and 5'-ADP. The Km value for 5'-IMP was 3.0 × 10-5 M. The enzyme was competitively inhibited by ATP and ADP, and the K1 values for ATP and ADP were 0.4 and 0.13μM, respectively." @default.
- W2323429265 created "2016-06-24" @default.
- W2323429265 creator A5047706758 @default.
- W2323429265 creator A5055807109 @default.
- W2323429265 date "1992-01-01" @default.
- W2323429265 modified "2023-09-27" @default.
- W2323429265 title "Purification and Properties of 5'-Nucleotidase from Snapper Muscle." @default.
- W2323429265 doi "https://doi.org/10.2331/suisan.58.1905" @default.
- W2323429265 hasPublicationYear "1992" @default.
- W2323429265 type Work @default.
- W2323429265 sameAs 2323429265 @default.
- W2323429265 citedByCount "1" @default.
- W2323429265 crossrefType "journal-article" @default.
- W2323429265 hasAuthorship W2323429265A5047706758 @default.
- W2323429265 hasAuthorship W2323429265A5055807109 @default.
- W2323429265 hasBestOaLocation W23234292651 @default.
- W2323429265 hasConcept C101660505 @default.
- W2323429265 hasConcept C116084860 @default.
- W2323429265 hasConcept C179247698 @default.
- W2323429265 hasConcept C181199279 @default.
- W2323429265 hasConcept C185592680 @default.
- W2323429265 hasConcept C193363816 @default.
- W2323429265 hasConcept C2777913276 @default.
- W2323429265 hasConcept C2779881004 @default.
- W2323429265 hasConcept C28212737 @default.
- W2323429265 hasConcept C43617362 @default.
- W2323429265 hasConcept C55493867 @default.
- W2323429265 hasConceptScore W2323429265C101660505 @default.
- W2323429265 hasConceptScore W2323429265C116084860 @default.
- W2323429265 hasConceptScore W2323429265C179247698 @default.
- W2323429265 hasConceptScore W2323429265C181199279 @default.
- W2323429265 hasConceptScore W2323429265C185592680 @default.
- W2323429265 hasConceptScore W2323429265C193363816 @default.
- W2323429265 hasConceptScore W2323429265C2777913276 @default.
- W2323429265 hasConceptScore W2323429265C2779881004 @default.
- W2323429265 hasConceptScore W2323429265C28212737 @default.
- W2323429265 hasConceptScore W2323429265C43617362 @default.
- W2323429265 hasConceptScore W2323429265C55493867 @default.
- W2323429265 hasIssue "10" @default.
- W2323429265 hasLocation W23234292651 @default.
- W2323429265 hasOpenAccess W2323429265 @default.
- W2323429265 hasPrimaryLocation W23234292651 @default.
- W2323429265 hasRelatedWork W1440031586 @default.
- W2323429265 hasRelatedWork W1578268344 @default.
- W2323429265 hasRelatedWork W1649084527 @default.
- W2323429265 hasRelatedWork W1989751382 @default.
- W2323429265 hasRelatedWork W2016077305 @default.
- W2323429265 hasRelatedWork W2025964199 @default.
- W2323429265 hasRelatedWork W2028233503 @default.
- W2323429265 hasRelatedWork W2072352214 @default.
- W2323429265 hasRelatedWork W2076636492 @default.
- W2323429265 hasRelatedWork W2374065000 @default.
- W2323429265 hasVolume "58" @default.
- W2323429265 isParatext "false" @default.
- W2323429265 isRetracted "false" @default.
- W2323429265 magId "2323429265" @default.
- W2323429265 workType "article" @default.