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• W2323444706 abstract "Event Abstract Back to Event Engineering scaffolds for bone tissue engineering using cell-laden gelatin methacrylate hydrogels Pranav Soman1, Stephen W. Sawyer1, Lucas D. Albrecht1, Megan Oest2 and Bryan S. Margulies2 1 Syracuse University, Biomedical and Chemical Engineering, United States 2 SUNY Upstate Medical University, Department of Cell and Developmental Biology, United States One of the more challenging aspects of tissue engineering involves the construction of scaffolds that are both clinically relevant and able to promote the creation of new organs de novo, but no single technique has emerged as a complete solution[1],[2]. Recently, collagen derived biomaterials such as gelatin methacrylate (GelMA) have been extensively used in tissue engineering applications because of their similarities to the native extracellular matrix (ECM), their readily variable elastic moduli, and their compatibility with cellular functionality. As a result, 3D cross-linkable GelMA scaffolds have emerged as ideal matrices for cell encapsulation studies and the creation of biomimetic scaffolds[3]-[11]. In this current study, bone-like human osteosarcoma cells (Saos-2; ATCC) are encapsulated inside GelMA hydrogels of varying stiffness and induced to form mineral in order to determine the empirical relationship between bone formation and scaffold moduli. Using a recently reported surface tension-based fabrication method for encapsulation of cells[11], Saos-2 cells transfected with green fluorescent protein (GFP) were seeded inside three different types of GelMA spheres (7%, 10%, and 15% w/v) approximately 5mm in diameter. Low seeding densities were used to increase interactions between the cells and their microenvironment and reduce cell-cell interactions. Following encapsulation, the cells were induced to form mineral, sectioned, and analyzed in order to determine differences between cellular morphology and establish empirical relationships between mineral formation and scaffold stiffness. Computed tomography was also used to further image and quantify mineral in induced samples Distinct differences between cell viability, cell migration, cell morphology, and mineral formation were found in the three different types of scaffolds. Softer scaffolds were shown to provide a more cell friendly microenvironment that promoted proliferation and migration, while harder scaffolds provided a more vibrant environment for proper bone-mineral formation. As shown in Figure 1, while cells were able to function in both soft and stiff matrices, the different environments caused pronounced variances in cell behavior. In 7% GelMA cells remained dispersed throughout the scaffold and were shown to produced mineral randomly (black dots), while cells in 15% GelMA where shown to migrate towards the periphery and produce more uniform bone. Future work will involve the integration of 3D printed polycaprolactone (PCL) frames as shown in Figure 1, with cell-laden hydrogel and have been recently demonstrated within our laboratory. The Soft Interfaces Integrative Graduate Education and Research Traineeship (IGERT) at Syracuse UniversityReferences:[1] Sachlos, E. & Czernuszka, J.T. Making tissue engineering scaffolds work. Review on the application of solid freeform fabrication technology to the production of tissue engineering scaffolds. European Cells and Materials 2003;5:29-40.[2] Drury, J. & Mooney, D. Hydrogels for tissue engineering: scaffold design variables and applications. Biomaterials 2003;24:4337-51.[3] Annabi N, Tamayol A, Uquillas JA, Akbari M, Bertassoni LE, Cha C, et al. 25th Anniversary Article: Rational design and applications of hydrogels in regenerative medicine. Advanced Materials 2014;26:85-124.[4] Antoniac I, Billiet T, Vandenhaute M, Schelfhout J, Vlierberghe S, Dubruel P. Exploring the Future of Hydrogels in Rapid Prototyping: A Review on Current Trends and Limitations. Biologically Responsive Biomaterials for Tissue Engineering: Springer New York; 2013. p. 201-49.[5] Khademhosseini A, Langer R. Microengineered hydrogels for tissue engineering. Biomaterials 2007;28:5087-92.[6] Lee K, Mooney D. Hydrogels for tissue engineering. Chem Rev 2001;101:1869-79.[7] Slaughter BV, Khurshid SS, Fisher OZ, Khademhosseini A, Peppas NA. Hydrogels in regenerative medicine. Advanced Materials 2009;21:3307-29.[8] Tibbitt MW, Anseth KS. Hydrogels as extracellular matrix mimics for 3D cell culture. Biotechnology and Bioengineering 2009;103:655-63.[9] Kloxin AM, Tibbitt MW, Anseth KS. Synthesis of photodegradable hydrogels as dynamically tunable cell culture platforms. Nature protocols 2010;5:1867-87.[10] Lewis KJ, Anseth KS. Hydrogel scaffolds to study cell biology in four dimensions. MRS bulletin 2013;38:260-8.[11] Pradhan S, Chaudhury CS, Lipke EA. Dual-Phase, Surface Tension-Based Fabrication Method for Generation of Tumor Millibeads. Langmuir 2014; 30:3817-25. Keywords: Bone Regeneration, Hydrogel, biomaterial, 3D scaffold Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomaterials in constructing tissue substitutes Citation: Soman P, Sawyer SW, Albrecht LD, Oest M and Margulies BS (2016). Engineering scaffolds for bone tissue engineering using cell-laden gelatin methacrylate hydrogels. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02569 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Pranav Soman Stephen W Sawyer Lucas D Albrecht Megan Oest Bryan S Margulies Google Pranav Soman Stephen W Sawyer Lucas D Albrecht Megan Oest Bryan S Margulies Google Scholar Pranav Soman Stephen W Sawyer Lucas D Albrecht Megan Oest Bryan S Margulies PubMed Pranav Soman Stephen W Sawyer Lucas D Albrecht Megan Oest Bryan S Margulies Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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