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- W2323573806 abstract "The toxin RelE is a ribosome-dependent endoribonuclease implicated in diverse cellular processes, including persistence. During amino acid starvation, RelE inhibits translation by cleaving ribosomal A-site mRNA. Although RelE is structurally similar to other microbial endoribonucleases, the active-site amino acid composition differs substantially and lacks obvious candidates for general acid–base functionality. Highly conserved RelE residues (Lys52, Lys54, Arg61, Arg81, and Tyr87) surround the mRNA scissile phosphate, and specific 16S rRNA contacts further contribute to substrate positioning. We used a single-turnover kinetic assay to evaluate the catalytic importance of individual residues in the RelE active site. Within the context of the ribosome, RelE rapidly cleaves A-site mRNA at a rate similar to those of traditional ribonucleases. Single-turnover rate constants decreased between 102- and 106-fold for the RelE active-site mutants of Lys52, Lys54, Arg61, and Arg81. RelE may principally promote catalysis via transition-state charge stabilization and leaving-group protonation, in addition to achieving in-line substrate positioning in cooperation with the ribosome. This kinetic analysis complements structural information to provide a foundation for understanding the molecular mechanism of this atypical endoribonuclease." @default.
- W2323573806 created "2016-06-24" @default.
- W2323573806 creator A5023994701 @default.
- W2323573806 creator A5039902482 @default.
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- W2323573806 date "2013-11-19" @default.
- W2323573806 modified "2023-09-23" @default.
- W2323573806 title "Bacterial Toxin RelE: A Highly Efficient Ribonuclease with Exquisite Substrate Specificity Using Atypical Catalytic Residues" @default.
- W2323573806 cites W1500367626 @default.
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- W2323573806 cites W1535452251 @default.
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- W2323573806 cites W1963863783 @default.
- W2323573806 cites W1964051028 @default.
- W2323573806 cites W1971182469 @default.
- W2323573806 cites W1974000520 @default.
- W2323573806 cites W1978655326 @default.
- W2323573806 cites W1978737991 @default.
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- W2323573806 cites W1992735817 @default.
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- W2323573806 cites W2003125843 @default.
- W2323573806 cites W2007735374 @default.
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- W2323573806 doi "https://doi.org/10.1021/bi401325c" @default.
- W2323573806 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3910103" @default.
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- W2323573806 hasPublicationYear "2013" @default.
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