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- W2323728049 abstract "Lysozyme is a protein found in egg white, tears, saliva, and other secretions. As a marketable natural alternative to preservatives, lysozyme can act as a natural antibiotic. In this study, we have isolated Bacillus licheniformis TIB320 from soil, which contains a lysozyme gene with various features. We have cloned and expressed the lysozyme in E. coli. The antimicrobial activity of the lysozyme showed that it had a broad antimicrobial spectrum against several standard strains. The lysozyme could maintain efficient activities in a pH range between 3 and 9 and from 20°C to 60°C, respectively. The lysozyme was resistant to pepsin and trypsin to some extent at 40°C. Production of the lysozyme was optimized by using various expression strategies in B. subtilis WB800. The lysozyme from B. licheniformis TIB320 will be promising as a food or feed additive." @default.
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- W2323728049 date "2014-10-28" @default.
- W2323728049 modified "2023-10-18" @default.
- W2323728049 title "Cloning, Characterization, and Production of a Novel Lysozyme by Different Expression Hosts" @default.
- W2323728049 doi "https://doi.org/10.4014/jmb.1404.04039" @default.
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