FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2323786391> ?p ?o ?g. }

• W2323786391 endingPage "3811" @default.
• W2323786391 startingPage "3803" @default.
• W2323786391 abstract "The lacLM genes from Lactobacillus sakei Lb790, encoding a heterodimeric β-galactosidase that belongs to glycoside hydrolase family GH2, were cloned and heterologously expressed in Escherichia coli. Subsequently, the recombinant β-galactosidase LacLM was purified to apparent homogeneity and characterized. The enzyme is a β-galactosidase with narrow substrate specificity because o-nitrophenyl-β-d-galactopyranoside (oNPG) was efficiently hydrolyzed, whereas various structurally related oNP analogues were not. The Km and kcat values for oNPG and lactose were 0.6 mM and 180 s−1 and 20 mM and 43 s−1, respectively. The enzyme is inhibited competitively by its two end-products d-galactose and d-glucose (Ki values of 180 and 475 mM, respectively). As judged by the ratio of the inhibition constant to the Michaelis constant, Ki/Km, this inhibition is only very moderate and much less pronounced than for other microbial β-galactosidases. β-Galactosidase from L. sakei possesses high transgalactosylation activity and was used for the synthesis of galacto-oligosaccharides (GalOS), employing lactose at a concentration of 215 g/L. The maximum GalOS yield was 41% (w/w) of total sugars at 77% lactose conversion and contained mainly non-lactose disaccharides, trisaccharides, and tetrasaccharides with approximately 38, 57, and 5% of total GalOS formed, respectively. The enzyme showed a strong preference for the formation of β-(1→6)-linked transgalactosylation products, whereas β-(1→3)-linked compounds were formed to a lesser extent and β-(1→4)-linked reaction products could not be detected." @default.
• W2323786391 created "2016-06-24" @default.
• W2323786391 creator A5003082351 @default.
• W2323786391 creator A5046941318 @default.
• W2323786391 creator A5058308244 @default.
• W2323786391 creator A5061925350 @default.
• W2323786391 creator A5067101387 @default.
• W2323786391 creator A5081657582 @default.
• W2323786391 creator A5082959067 @default.
• W2323786391 date "2011-03-25" @default.
• W2323786391 modified "2023-10-02" @default.
• W2323786391 title "Characterization of a Heterodimeric GH2 β-Galactosidase from Lactobacillus sakei Lb790 and Formation of Prebiotic Galacto-oligosaccharides" @default.
• W2323786391 cites W1985785054 @default.
• W2323786391 cites W1986017237 @default.
• W2323786391 cites W1995570514 @default.
• W2323786391 cites W1999531030 @default.
• W2323786391 cites W2000525220 @default.
• W2323786391 cites W2005318455 @default.
• W2323786391 cites W2011141815 @default.
• W2323786391 cites W2015224932 @default.
• W2323786391 cites W2021651633 @default.
• W2323786391 cites W2029403879 @default.
• W2323786391 cites W2037172439 @default.
• W2323786391 cites W2041485793 @default.
• W2323786391 cites W2042999004 @default.
• W2323786391 cites W2045545069 @default.
• W2323786391 cites W2046786452 @default.
• W2323786391 cites W2050996471 @default.
• W2323786391 cites W2053931123 @default.
• W2323786391 cites W2058791628 @default.
• W2323786391 cites W2061344056 @default.
• W2323786391 cites W2062552121 @default.
• W2323786391 cites W2068634673 @default.
• W2323786391 cites W2075559014 @default.
• W2323786391 cites W2076058236 @default.
• W2323786391 cites W2077057222 @default.
• W2323786391 cites W2077133508 @default.
• W2323786391 cites W2077792616 @default.
• W2323786391 cites W2088685376 @default.
• W2323786391 cites W2096299972 @default.
• W2323786391 cites W2117065897 @default.
• W2323786391 cites W2119173647 @default.
• W2323786391 cites W2122254428 @default.
• W2323786391 cites W2130558603 @default.
• W2323786391 cites W2131666346 @default.
• W2323786391 cites W2151251013 @default.
• W2323786391 cites W2155836215 @default.
• W2323786391 cites W2160210736 @default.
• W2323786391 cites W2165875368 @default.
• W2323786391 cites W4249075929 @default.
• W2323786391 cites W69622561 @default.
• W2323786391 cites W9345227 @default.
• W2323786391 doi "https://doi.org/10.1021/jf103832q" @default.
• W2323786391 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21405014" @default.
• W2323786391 hasPublicationYear "2011" @default.
• W2323786391 type Work @default.
• W2323786391 sameAs 2323786391 @default.
• W2323786391 citedByCount "58" @default.
• W2323786391 countsByYear W23237863912012 @default.
• W2323786391 countsByYear W23237863912013 @default.
• W2323786391 countsByYear W23237863912014 @default.
• W2323786391 countsByYear W23237863912015 @default.
• W2323786391 countsByYear W23237863912016 @default.
• W2323786391 countsByYear W23237863912017 @default.
• W2323786391 countsByYear W23237863912018 @default.
• W2323786391 countsByYear W23237863912019 @default.
• W2323786391 countsByYear W23237863912020 @default.
• W2323786391 countsByYear W23237863912021 @default.
• W2323786391 countsByYear W23237863912022 @default.
• W2323786391 countsByYear W23237863912023 @default.
• W2323786391 crossrefType "journal-article" @default.
• W2323786391 hasAuthorship W2323786391A5003082351 @default.
• W2323786391 hasAuthorship W2323786391A5046941318 @default.
• W2323786391 hasAuthorship W2323786391A5058308244 @default.
• W2323786391 hasAuthorship W2323786391A5061925350 @default.
• W2323786391 hasAuthorship W2323786391A5067101387 @default.
• W2323786391 hasAuthorship W2323786391A5081657582 @default.
• W2323786391 hasAuthorship W2323786391A5082959067 @default.
• W2323786391 hasConcept C100544194 @default.
• W2323786391 hasConcept C104317684 @default.
• W2323786391 hasConcept C181199279 @default.
• W2323786391 hasConcept C185592680 @default.
• W2323786391 hasConcept C196032511 @default.
• W2323786391 hasConcept C2777249184 @default.
• W2323786391 hasConcept C2777346074 @default.
• W2323786391 hasConcept C2778173381 @default.
• W2323786391 hasConcept C2778724459 @default.
• W2323786391 hasConcept C2780120296 @default.
• W2323786391 hasConcept C2780206646 @default.
• W2323786391 hasConcept C2780338318 @default.
• W2323786391 hasConcept C41183919 @default.
• W2323786391 hasConcept C43053761 @default.
• W2323786391 hasConcept C547475151 @default.
• W2323786391 hasConcept C55493867 @default.
• W2323786391 hasConcept C56856141 @default.
• W2323786391 hasConcept C71240020 @default.
• W2323786391 hasConcept C94412978 @default.
• W2323786391 hasConceptScore W2323786391C100544194 @default.

• next