FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2324004869> ?p ?o ?g. }

• W2324004869 endingPage "1902" @default.
• W2324004869 startingPage "1895" @default.
• W2324004869 abstract "Polyethylenimine (PEI) is a cationic DNA condensing polymer that facilitates gene transfer into the mammalian cells. The highest gene transfer with branched PEI is obtained at high nitrogen/phosphate (N/P) ratios with free PEI present. The small molecular weight PEI alone is not able to mediate DNA transfection. Here, we used recently developed time-resolved fluorescence spectroscopic method to study the mechanism of PEI-DNA complex formation and to investigate how free PEI, mean molecular weight, and branching of PEI affect the complexes. Analysis of fluorescence lifetimes and time-resolved spectra revealed that for both linear and branched high-molecular-weight PEI the complexation takes place in two steps, but the small-molecular-weight branched PEI complexed DNA at a single step. According to the binding constants obtained from time-resolved spectroscopic measurements, the affinity of N/P complexation per nitrogen atom is highest for LPEI and weakest for BPEI, whereas SPEI-DNA complexation showed intermediate values. Thus, the binding constant alone does not give adequate measure for transfection efficiency. On the other hand, the presence of intermediate states during the polyplex formation seems to be favorable for the gene transfection. Free PEI had no impact on the physical state of PEI-DNA complexes, even though it was essential for gene transfection in the cell culture. In conclusion, the molecular size and topology of PEI have direct influence on the DNA complexation but the free PEI does not. Free PEI must facilitate transfection at the cellular level and not via indirect effects on the PEI-DNA complexes." @default.
• W2324004869 created "2016-06-24" @default.
• W2324004869 creator A5024442454 @default.
• W2324004869 creator A5029010624 @default.
• W2324004869 creator A5053032683 @default.
• W2324004869 creator A5065810893 @default.
• W2324004869 creator A5066687090 @default.
• W2324004869 creator A5082738010 @default.
• W2324004869 date "2011-02-03" @default.
• W2324004869 modified "2023-09-30" @default.
• W2324004869 title "Role of Polyplex Intermediate Species on Gene Transfer Efficiency: Polyethylenimine−DNA Complexes and Time-Resolved Fluorescence Spectroscopy" @default.
• W2324004869 cites W1577071491 @default.
• W2324004869 cites W1968113316 @default.
• W2324004869 cites W1981206638 @default.
• W2324004869 cites W1985040895 @default.
• W2324004869 cites W1987163952 @default.
• W2324004869 cites W1989420688 @default.
• W2324004869 cites W1993081501 @default.
• W2324004869 cites W1994354502 @default.
• W2324004869 cites W1997993853 @default.
• W2324004869 cites W2009508594 @default.
• W2324004869 cites W2010655741 @default.
• W2324004869 cites W2011682937 @default.
• W2324004869 cites W2013156289 @default.
• W2324004869 cites W2015636882 @default.
• W2324004869 cites W2041153298 @default.
• W2324004869 cites W2058612400 @default.
• W2324004869 cites W2075049718 @default.
• W2324004869 cites W2080071319 @default.
• W2324004869 cites W2080648192 @default.
• W2324004869 cites W2086274688 @default.
• W2324004869 cites W2128314443 @default.
• W2324004869 cites W2163792954 @default.
• W2324004869 cites W2163921171 @default.
• W2324004869 cites W2170553025 @default.
• W2324004869 cites W254513662 @default.
• W2324004869 doi "https://doi.org/10.1021/jp109984c" @default.
• W2324004869 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21291220" @default.
• W2324004869 hasPublicationYear "2011" @default.
• W2324004869 type Work @default.
• W2324004869 sameAs 2324004869 @default.
• W2324004869 citedByCount "32" @default.
• W2324004869 countsByYear W23240048692012 @default.
• W2324004869 countsByYear W23240048692013 @default.
• W2324004869 countsByYear W23240048692014 @default.
• W2324004869 countsByYear W23240048692015 @default.
• W2324004869 countsByYear W23240048692016 @default.
• W2324004869 countsByYear W23240048692017 @default.
• W2324004869 countsByYear W23240048692018 @default.
• W2324004869 countsByYear W23240048692019 @default.
• W2324004869 countsByYear W23240048692020 @default.
• W2324004869 countsByYear W23240048692021 @default.
• W2324004869 countsByYear W23240048692022 @default.
• W2324004869 countsByYear W23240048692023 @default.
• W2324004869 crossrefType "journal-article" @default.
• W2324004869 hasAuthorship W2324004869A5024442454 @default.
• W2324004869 hasAuthorship W2324004869A5029010624 @default.
• W2324004869 hasAuthorship W2324004869A5053032683 @default.
• W2324004869 hasAuthorship W2324004869A5065810893 @default.
• W2324004869 hasAuthorship W2324004869A5066687090 @default.
• W2324004869 hasAuthorship W2324004869A5082738010 @default.
• W2324004869 hasConcept C104317684 @default.
• W2324004869 hasConcept C121332964 @default.
• W2324004869 hasConcept C12554922 @default.
• W2324004869 hasConcept C158711907 @default.
• W2324004869 hasConcept C183882617 @default.
• W2324004869 hasConcept C185592680 @default.
• W2324004869 hasConcept C188027245 @default.
• W2324004869 hasConcept C204389451 @default.
• W2324004869 hasConcept C2775925850 @default.
• W2324004869 hasConcept C54009773 @default.
• W2324004869 hasConcept C552990157 @default.
• W2324004869 hasConcept C55493867 @default.
• W2324004869 hasConcept C62520636 @default.
• W2324004869 hasConcept C86803240 @default.
• W2324004869 hasConcept C91881484 @default.
• W2324004869 hasConceptScore W2324004869C104317684 @default.
• W2324004869 hasConceptScore W2324004869C121332964 @default.
• W2324004869 hasConceptScore W2324004869C12554922 @default.
• W2324004869 hasConceptScore W2324004869C158711907 @default.
• W2324004869 hasConceptScore W2324004869C183882617 @default.
• W2324004869 hasConceptScore W2324004869C185592680 @default.
• W2324004869 hasConceptScore W2324004869C188027245 @default.
• W2324004869 hasConceptScore W2324004869C204389451 @default.
• W2324004869 hasConceptScore W2324004869C2775925850 @default.
• W2324004869 hasConceptScore W2324004869C54009773 @default.
• W2324004869 hasConceptScore W2324004869C552990157 @default.
• W2324004869 hasConceptScore W2324004869C55493867 @default.
• W2324004869 hasConceptScore W2324004869C62520636 @default.
• W2324004869 hasConceptScore W2324004869C86803240 @default.
• W2324004869 hasConceptScore W2324004869C91881484 @default.
• W2324004869 hasIssue "8" @default.
• W2324004869 hasLocation W23240048691 @default.
• W2324004869 hasLocation W23240048692 @default.
• W2324004869 hasOpenAccess W2324004869 @default.
• W2324004869 hasPrimaryLocation W23240048691 @default.
• W2324004869 hasRelatedWork W2048934762 @default.
• W2324004869 hasRelatedWork W2061409101 @default.

• next