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- W2324069964 abstract "Bioorthogonal reactions are of high interest in biosciences as they allow the introduction of fluorescent dyes, affinity tags, or other unnatural moieties into biomolecules. The site-specific attachment of two or more different labels is particularly demanding and typically requires laborious multistep syntheses. Here, we report that the most popular cycloaddition in bioconjugation, the copper-catalyzed azide–alkyne click reaction (CuAAC), is fully orthogonal to the inverse electron-demand Diels–Alder reaction (DAinv). We demonstrate that both bioorthogonal reactions can be conducted concurrently in a one-pot reaction by just mixing all components. Orthogonality has been established even for highly reactive trans-cyclooctene-based dienophiles (with rate constants up to 380 000 M–1 s–1). These properties allow for the convenient site-specific one-step preparation of oligonucleotide FRET probes and related reporters needed in cellular biology and biophysical chemistry." @default.
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- W2324069964 date "2012-06-25" @default.
- W2324069964 modified "2023-10-18" @default.
- W2324069964 title "Site-Specific One-Pot Dual Labeling of DNA by Orthogonal Cycloaddition Chemistry" @default.
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- W2324069964 doi "https://doi.org/10.1021/bc300181n" @default.
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