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- W2324334529 abstract "Recent work has demonstrated that the receptor-mediated signaling system in chemotactic amoeboid cells shows typical properties of an excitable system. Here, we delivered spatially confined stimuli of the chemoattractant cAMP to the membrane of differentiated Dictyostelium discoideum cells to investigate whether localized receptor stimuli can induce the spreading of excitable waves in the G-protein dependent signal transduction system. By imaging the spatiotemporal dynamics of fluorescent markers for PIP3, PTEN, and filamentous actin, we observed that the activity of the signaling pathway remained spatially confined to the stimulated membrane region. Neighboring parts of the membrane were not excited and no receptor-initiated spatial spreading of excitation waves was observed. To generate localized cAMP stimuli, either particles that carried covalently bound cAMP molecules on their surface were brought into contact with the cell, or a patch of the cell membrane was aspirated into a glass micropipette to shield this patch against freely diffusing cAMP molecules in the surrounding medium. Additionally, also the binding site of the cAMP receptor was probed with different surface-immobilized cAMP molecules, confirming results from earlier ligand binding studies." @default.
- W2324334529 created "2016-06-24" @default.
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- W2324334529 date "2014-01-01" @default.
- W2324334529 modified "2023-09-27" @default.
- W2324334529 title "Signaling in chemotactic amoebae remains spatially confined to stimulated membrane regions" @default.
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- W2324334529 doi "https://doi.org/10.1242/jcs.161133" @default.
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