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- W2324336429 abstract "Abstract The green microalga Botryococcus braunii is considered a promising biofuel feedstock producer due to its prodigious accumulation of hydrocarbon oils that can be converted into fuels. B. braunii Race L produces the C 40 tetraterpenoid hydrocarbon lycopadiene via an uncharacterized biosynthetic pathway. Structural similarities suggest this pathway follows a biosynthetic mechanism analogous to that of C 30 squalene. Confirming this hypothesis, the current study identifies C 20 geranylgeranyl diphosphate (GGPP) as a precursor for lycopaoctaene biosynthesis, the first committed intermediate in the production of lycopadiene. Two squalene synthase ( SS )-like complementary DNAs are identified in race L with one encoding a true SS and the other encoding an enzyme with lycopaoctaene synthase (LOS) activity. Interestingly, LOS uses alternative C 15 and C 20 prenyl diphosphate substrates to produce combinatorial hybrid hydrocarbons, but almost exclusively uses GGPP in vivo . This discovery highlights how SS enzyme diversification results in the production of specialized tetraterpenoid oils in race L of B. braunii ." @default.
- W2324336429 created "2016-06-24" @default.
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- W2324336429 date "2016-04-06" @default.
- W2324336429 modified "2023-10-15" @default.
- W2324336429 title "A squalene synthase-like enzyme initiates production of tetraterpenoid hydrocarbons in Botryococcus braunii Race L" @default.
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- W2324336429 doi "https://doi.org/10.1038/ncomms11198" @default.
- W2324336429 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4823828" @default.
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