FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2324586783> ?p ?o ?g. }

• W2324586783 endingPage "186" @default.
• W2324586783 startingPage "179" @default.
• W2324586783 abstract "Activation of the endothelium is a critical event in the process of inflammation and is associated with the production of chemokines.To evaluate the proinflammatory cytokine-induced chemokine repertoire of human coronary-artery endothelial cells (HCAEC) both at the messenger RNA (mRNA) level and at protein level in direct comparison with that of human umbilical-vein endothelial cells (HUVEC).Human coronary-artery and human umbilical-vein endothelial cells were obtained commercially and experimental data were derived from cell cultures between passage levels 3 through 6. Supernatant fluids from cytokine [tumor necrosis factor-alpha (TNF-alpha), interleukin-1-alpha, and anti-TNF R55] stimulated endothelial cell cultures were used to study chemokine release. Sandwiched ELISA assays, obtained commercially, were used to estimate cell culture supernatant fluid levels of the selected chemokines: monocytic chemotactic protein-1, regulated upon activated normal T cells expressed and secreted, interleukin-8, transforming growth factor-beta-2 (TGF-beta2), and gamma interferon protein-10. Expression of messenger RNA was determined using selected labeled riboprobes (32P UTP) in a ribonuclease protection assay using total cellular mRNA.Upon in-vitro stimulation with TNF-alpha and interleukin-1-alpha, production of regulated-upon-activated-normal-T-cells expressed and secreted (RANTES) protein by HCAEC was significantly increased relative to that by HUVEC, the greatest effect being found with interleukin-1-alpha. The opposite effect, however, was noted for levels of monocytic-chemotactic-protein-1 protein, which were detected in HUVEC at significantly higher levels than they were in HCAEC challenged by those cytokines. Production of gamma interferon-inducible protein-10 (gammaIP-10) by HUVEC was induced by TNF-alpha and interleukin-1-alpha, whereas only a modest induction by interleukin-1-alpha was seen in HCAEC. TGF-beta-2 protein was constitutively expressed in HCAEC but not in HUVEC. Expression of mRNA was analyzed by the ribonuclease-protectionassay. RANTES mRNA was expressed in HCAEC from 3 h through 48 h after treatment with TNF-alpha, whereas only a modest induction of RANTES was expressed in HUVEC 24 h and 48 h after treatment with TNF-alpha. Monocytic-chemotactic-protein-1 mRNA was constitutively expressed by both types of cell, but the basal levels in HCAEC was significantly higher than in HUVEC. HCAEC constitutively expressed both TGF-beta-1 and TGF-beta-2 mRNA, whereas HUVEC constitutively expressed TGF-beta-1 only.Our data indicate that HCAEC and HUVEC express chemokines differently, which could contribute to or influence site-specific recruitment of subsets of leukocytes." @default.
• W2324586783 created "2016-06-24" @default.
• W2324586783 creator A5014714493 @default.
• W2324586783 creator A5039342410 @default.
• W2324586783 creator A5074387570 @default.
• W2324586783 creator A5082442093 @default.
• W2324586783 date "2001-05-01" @default.
• W2324586783 modified "2023-09-24" @default.
• W2324586783 title "Expression of chemokine by human coronary-artery and umbilical-vein endothelial cells and its regulation by inflammatory cytokines" @default.
• W2324586783 cites W116896294 @default.
• W2324586783 cites W1971169128 @default.
• W2324586783 cites W1978993962 @default.
• W2324586783 cites W1991720779 @default.
• W2324586783 cites W1992715029 @default.
• W2324586783 cites W1994853655 @default.
• W2324586783 cites W2005659566 @default.
• W2324586783 cites W2016366526 @default.
• W2324586783 cites W2043139614 @default.
• W2324586783 cites W2051836311 @default.
• W2324586783 cites W2094720821 @default.
• W2324586783 cites W4211264883 @default.
• W2324586783 doi "https://doi.org/10.1097/00019501-200105000-00004" @default.
• W2324586783 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11352074" @default.
• W2324586783 hasPublicationYear "2001" @default.
• W2324586783 type Work @default.
• W2324586783 sameAs 2324586783 @default.
• W2324586783 citedByCount "20" @default.
• W2324586783 countsByYear W23245867832012 @default.
• W2324586783 countsByYear W23245867832016 @default.
• W2324586783 countsByYear W23245867832017 @default.
• W2324586783 countsByYear W23245867832018 @default.
• W2324586783 countsByYear W23245867832021 @default.
• W2324586783 crossrefType "journal-article" @default.
• W2324586783 hasAuthorship W2324586783A5014714493 @default.
• W2324586783 hasAuthorship W2324586783A5039342410 @default.
• W2324586783 hasAuthorship W2324586783A5074387570 @default.
• W2324586783 hasAuthorship W2324586783A5082442093 @default.
• W2324586783 hasConcept C123012128 @default.
• W2324586783 hasConcept C126322002 @default.
• W2324586783 hasConcept C13373296 @default.
• W2324586783 hasConcept C153911025 @default.
• W2324586783 hasConcept C164027704 @default.
• W2324586783 hasConcept C17991360 @default.
• W2324586783 hasConcept C202751555 @default.
• W2324586783 hasConcept C203014093 @default.
• W2324586783 hasConcept C2776914184 @default.
• W2324586783 hasConcept C2776992346 @default.
• W2324586783 hasConcept C2777411675 @default.
• W2324586783 hasConcept C2778690821 @default.
• W2324586783 hasConcept C55493867 @default.
• W2324586783 hasConcept C68324877 @default.
• W2324586783 hasConcept C71924100 @default.
• W2324586783 hasConcept C86803240 @default.
• W2324586783 hasConceptScore W2324586783C123012128 @default.
• W2324586783 hasConceptScore W2324586783C126322002 @default.
• W2324586783 hasConceptScore W2324586783C13373296 @default.
• W2324586783 hasConceptScore W2324586783C153911025 @default.
• W2324586783 hasConceptScore W2324586783C164027704 @default.
• W2324586783 hasConceptScore W2324586783C17991360 @default.
• W2324586783 hasConceptScore W2324586783C202751555 @default.
• W2324586783 hasConceptScore W2324586783C203014093 @default.
• W2324586783 hasConceptScore W2324586783C2776914184 @default.
• W2324586783 hasConceptScore W2324586783C2776992346 @default.
• W2324586783 hasConceptScore W2324586783C2777411675 @default.
• W2324586783 hasConceptScore W2324586783C2778690821 @default.
• W2324586783 hasConceptScore W2324586783C55493867 @default.
• W2324586783 hasConceptScore W2324586783C68324877 @default.
• W2324586783 hasConceptScore W2324586783C71924100 @default.
• W2324586783 hasConceptScore W2324586783C86803240 @default.
• W2324586783 hasIssue "3" @default.
• W2324586783 hasLocation W23245867831 @default.
• W2324586783 hasLocation W23245867832 @default.
• W2324586783 hasOpenAccess W2324586783 @default.
• W2324586783 hasPrimaryLocation W23245867831 @default.
• W2324586783 hasRelatedWork W1714940412 @default.
• W2324586783 hasRelatedWork W1987697173 @default.
• W2324586783 hasRelatedWork W1989466429 @default.
• W2324586783 hasRelatedWork W2015390492 @default.
• W2324586783 hasRelatedWork W2043581300 @default.
• W2324586783 hasRelatedWork W2063438040 @default.
• W2324586783 hasRelatedWork W2073023687 @default.
• W2324586783 hasRelatedWork W2078320770 @default.
• W2324586783 hasRelatedWork W2156376884 @default.
• W2324586783 hasRelatedWork W2338547355 @default.
• W2324586783 hasVolume "12" @default.
• W2324586783 isParatext "false" @default.
• W2324586783 isRetracted "false" @default.
• W2324586783 magId "2324586783" @default.
• W2324586783 workType "article" @default.