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• W2324666444 abstract "A unique direct-view stereo electron microscope technique was used to visualize the structure and three-dimensional distributions of mitochondria in CHO cells in situ following hyperthermic treatments. Aberrations induced by various heating regimens were recorded. The protocol included a trypsin digestion that may have enhanced the expression of the initial heat damage. The developed damage was observed as increasing levels of mitochondrial distortion, swelling, and dissociation. Minimal damage was induced at 42 degrees C for exposures of up to 4 h, while significant damage was induced at 43 degrees C for exposures of more than 30 min and at 45 degrees C for exposures of more than 10 min. For moderate exposures, a partial recovery of mitochondrial integrity was observed when the heat treatment was followed by incubation at 37 degrees C for 24 h. Mitochondrial damage was related to the heat dose in that increasing treatment temperature resulted in greater damage, but when compared to cell survival the damage did not parallel cell killing under all time-temperature conditions." @default.
• W2324666444 created "2016-06-24" @default.
• W2324666444 creator A5027687837 @default.
• W2324666444 creator A5069890928 @default.
• W2324666444 date "1988-09-01" @default.
• W2324666444 modified "2023-09-25" @default.
• W2324666444 title "Ultrastructural Study of Mitochondrial Damage in CHO Cells Exposed to Hyperthermia" @default.
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• W2324666444 doi "https://doi.org/10.2307/3577292" @default.
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