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- W2324802794 abstract "The N-butyl methacrylate (N-b.m.) imbedding method described by Woodring and Cook (1962) was found to be the most satisfactory for our purposes of the many methods tried. Owing to the alternation of heavily sclerotized and membranous structures in larvae of eriopterine crane flies (Tipulidae: Diptera), specimens imbedded in paraffins or Paraplast of different melting points would collapse or the tissues would compress and crush as the microtome blade passed through them. The N-b.m. mixture was found to provide the necessary strength to maintain the form and structure of the head capsule. This method did, however, present one serious difficulty—bubbles frequently appeared both inside and outside the tissues being imbedded. With the modifications presented here, we feel this problem has been solved. These modifications include: (1) the method of infiltration of the head capsule, (2) the polymerization time with respect to temperature of the N-b.m. mixture (N-b.m. + accelerator + Tissuemat), and (3) placement of the polymerized plastic block in Paraplast instead of in Tissuemat." @default.
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- W2324802794 date "1966-01-01" @default.
- W2324802794 modified "2023-10-18" @default.
- W2324802794 title "Modifications of the N-butyl Methacrylate Imbedding Method as Applied to Insect Larval Head Capsules1" @default.
- W2324802794 doi "https://doi.org/10.1093/aesa/59.1.230" @default.
- W2324802794 hasPublicationYear "1966" @default.
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