FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2324806979> ?p ?o ?g. }

• W2324806979 abstract "Event Abstract Back to Event Micro-topographical surface features interfere with osteoblast cell signaling Caroline Moerke1, Susanne Staehlke1 and Barbara J. Nebe1 1 University Medical Center Rostock, Cell Biology, Germany Introduction: In cell-material interaction, knowledge of the dependence of cell behavior on topographical features is relevant for designing implant surfaces. Cells are sensitive to their underlying topography and especially micro-topography offers cues that evoke large ranges of cell responses. Human MG-63 osteoblastic cells growing on top of micro-pillared structures altered their actin organization and the changed cell architecture resulted in a decreased synthesis of extracellular matrix proteins collagen-I, bone sialo protein-2[1] and fibronectin as well as impaired ATP induced mobilization of intracellular calcium[2]. But the complex interplay between the topography-induced cell morphology and functional changes is not completely understood. Material and Methods: Regular geometric micro-pillared structures (diameters: width 5 µm; length 5 µm; height 5 µm) were used as artificial surfaces. The micro-pillared topography was produced by deep-reactive ion etching of silicon wafers and coated with 100 nm titanium. Human MG-63 osteoblastic cells (ATCC, CRL-1427) were grown for 24 and 96 h on the surfaces. Cellular morphology was analyzed by scanning electron microscopy (SEM), gene expression of alkaline phosphatase, caveolin-1 (Cav-1), collagen type 1 (Col1), fibronectin (FN) and osteocalcin by real time PCR, protein expression of Cav-1, phosphorylated Cav-1, Col1, FN and bone sialo protein 2 by western blot and catalase expression by luminex assay. Protein localization of Cav-1 and cholesterol was determined by confocal microscopy. Via fluorescence quantification by FACS, micro-plate reader of microscopy reactive oxygen species (ROS) generation, intercellular ATP/ADP amount and mitochondrial activity were determined. Results: Cav-1 and cholesterol were found to be clustered on top of the micro-pillars after 24 h and the SEM showed MG-63 osteoblasts engulfing the micro-pillars after 96 h. Gene expression of osteoblast marker genes was impaired after 24 h as well as the protein expression after 96 h of cells grown on the micro-pillars. The energy metabolism was disturbed by the micro-pillars. This was indicated by a higher mitochondrial activity and reduced intercellular ATP amounts; accompanied with higher ROS levels and protein expression of ROS composition enzymes. Discussion: Our recent experiments revealed an attempted caveolae-mediated phagocytosis of the surface-fixed micro-pillars by osteoblasts attended by increased energy demands resulting in higher cell stress, e.g. ROS generation. Phagocytosis is known to reorganize the actin cytoskeleton[3] as observed on the micro-pillared topography and is also characterized to be an energy-dependent process[4] and therefore the topography-triggered phagocytosis may lead to the observed impaired osteoblastic cell signaling. German Research Foundation (DFG) graduate school welisa (1505/2)References:[1] Matschegewski C, Staehlke S, Loeffler R, Lange R, Chai F, Kern DP, Beck U, Nebe BJ. Cell architecture – cell function dependencies on titanium arrays with regular geometry. Biomaterials 2010;31:5729-5740. doi:10.1016/j.biomaterials.2010.03.073[2] Staehlke S, Koertge A, Nebe B. Intracellular calcium dynamics dependent on defined microtopographical features of titanium. Biomaterials 2015,46:48-57. doi:10.1016/j.biomaterials.2014.12.016[3] May RC, Machesky LM. Phagocytosis and the actin cytoskeleton. J Cell Sci 2001;114:1061-1077.[4] Borregaard N, Herlin T. Energy metabolism of human neutrophil during phagocytosis. J Clin Invest 1982;70:550-557. Keywords: biomaterial, microstructure, Cell response, Cell functionality Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomaterials and cellular signaling Citation: Moerke C, Staehlke S and Nebe BJ (2016). Micro-topographical surface features interfere with osteoblast cell signaling. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02564 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Caroline Moerke Susanne Staehlke Barbara J Nebe Google Caroline Moerke Susanne Staehlke Barbara J Nebe Google Scholar Caroline Moerke Susanne Staehlke Barbara J Nebe PubMed Caroline Moerke Susanne Staehlke Barbara J Nebe Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
• W2324806979 created "2016-06-24" @default.
• W2324806979 creator A5040919180 @default.
• W2324806979 creator A5041508467 @default.
• W2324806979 creator A5065400407 @default.
• W2324806979 date "2016-01-01" @default.
• W2324806979 modified "2023-10-14" @default.
• W2324806979 title "Micro-topographical surface features interfere with osteoblast cell signaling" @default.
• W2324806979 cites W1927734345 @default.
• W2324806979 cites W2023596226 @default.
• W2324806979 cites W2031445285 @default.
• W2324806979 cites W2151593963 @default.
• W2324806979 doi "https://doi.org/10.3389/conf.fbioe.2016.01.02564" @default.
• W2324806979 hasPublicationYear "2016" @default.
• W2324806979 type Work @default.
• W2324806979 sameAs 2324806979 @default.
• W2324806979 citedByCount "0" @default.
• W2324806979 crossrefType "journal-article" @default.
• W2324806979 hasAuthorship W2324806979A5040919180 @default.
• W2324806979 hasAuthorship W2324806979A5041508467 @default.
• W2324806979 hasAuthorship W2324806979A5065400407 @default.
• W2324806979 hasBestOaLocation W23248069791 @default.
• W2324806979 hasConcept C12554922 @default.
• W2324806979 hasConcept C131075544 @default.
• W2324806979 hasConcept C1491633281 @default.
• W2324806979 hasConcept C160160445 @default.
• W2324806979 hasConcept C181199279 @default.
• W2324806979 hasConcept C185592680 @default.
• W2324806979 hasConcept C189165786 @default.
• W2324806979 hasConcept C202751555 @default.
• W2324806979 hasConcept C2776432469 @default.
• W2324806979 hasConcept C2778260815 @default.
• W2324806979 hasConcept C55493867 @default.
• W2324806979 hasConcept C86492073 @default.
• W2324806979 hasConcept C86803240 @default.
• W2324806979 hasConcept C95444343 @default.
• W2324806979 hasConceptScore W2324806979C12554922 @default.
• W2324806979 hasConceptScore W2324806979C131075544 @default.
• W2324806979 hasConceptScore W2324806979C1491633281 @default.
• W2324806979 hasConceptScore W2324806979C160160445 @default.
• W2324806979 hasConceptScore W2324806979C181199279 @default.
• W2324806979 hasConceptScore W2324806979C185592680 @default.
• W2324806979 hasConceptScore W2324806979C189165786 @default.
• W2324806979 hasConceptScore W2324806979C202751555 @default.
• W2324806979 hasConceptScore W2324806979C2776432469 @default.
• W2324806979 hasConceptScore W2324806979C2778260815 @default.
• W2324806979 hasConceptScore W2324806979C55493867 @default.
• W2324806979 hasConceptScore W2324806979C86492073 @default.
• W2324806979 hasConceptScore W2324806979C86803240 @default.
• W2324806979 hasConceptScore W2324806979C95444343 @default.
• W2324806979 hasLocation W23248069791 @default.
• W2324806979 hasOpenAccess W2324806979 @default.
• W2324806979 hasPrimaryLocation W23248069791 @default.
• W2324806979 hasRelatedWork W1568198266 @default.
• W2324806979 hasRelatedWork W1992848813 @default.
• W2324806979 hasRelatedWork W2000359966 @default.
• W2324806979 hasRelatedWork W2028553359 @default.
• W2324806979 hasRelatedWork W2042186930 @default.
• W2324806979 hasRelatedWork W2062037888 @default.
• W2324806979 hasRelatedWork W2068520348 @default.
• W2324806979 hasRelatedWork W2167287018 @default.
• W2324806979 hasRelatedWork W2313637060 @default.
• W2324806979 hasRelatedWork W2552226920 @default.
• W2324806979 hasVolume "4" @default.
• W2324806979 isParatext "false" @default.
• W2324806979 isRetracted "false" @default.
• W2324806979 magId "2324806979" @default.
• W2324806979 workType "article" @default.