Matches in SemOpenAlex for { <https://semopenalex.org/work/W2324836477> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2324836477 endingPage "4595" @default.
- W2324836477 startingPage "4589" @default.
- W2324836477 abstract "Washing processes, essential in most heterogeneous labeled assays, have been a big hurdle in simplifying the detection procedure and reducing assay time. Nevertheless, less attention has been paid to washing-free heterogeneous labeled assays. We report a purely washing-free immunosensor that allows fast, sensitive, and single-step detection of prostate-specific antigen in serum with low interference. Proximity-dependent electron mediation of ferrocenemethanol (Fc) between an indium-tin oxide (ITO) electrode and a glucose-oxidase (GOx) label allows us to discriminate between a bound and an unbound label: a bound label offers faster electron mediation than an unbound one. The electrooxidation of Fc at a low applied potential (0.13 V vs Ag/AgCl) and a low electrocatalytic ITO electrode and the oxidation of l-ascorbic acid by l-ascorbate oxidase minimize the effect of the interfering species. With a high concentration of glucose (200 mM), the signal and background levels are hardly dependent on the glucose-concentration variation in the sample. The washing-free immunosensor can detect a concentration of ca. 1 pg/mL for mouse IgG in phosphate-buffered saline and a concentration of ca. 10 pg/mL for prostate-specific antigen spiked in female serum after an incubation period of 10 min. The concentrations measured with actual clinical serum samples are in good agreement with the concentrations measured with a commercial instrument, which renders the washing-free heterogeneous immunosensor appealing for practical use." @default.
- W2324836477 created "2016-06-24" @default.
- W2324836477 creator A5035405996 @default.
- W2324836477 creator A5073511552 @default.
- W2324836477 creator A5075509015 @default.
- W2324836477 creator A5090892663 @default.
- W2324836477 date "2014-04-23" @default.
- W2324836477 modified "2023-10-14" @default.
- W2324836477 title "Washing-Free Heterogeneous Immunosensor Using Proximity-Dependent Electron Mediation between an Enzyme Label and an Electrode" @default.
- W2324836477 cites W1970327912 @default.
- W2324836477 cites W1973179890 @default.
- W2324836477 cites W1977328354 @default.
- W2324836477 cites W2011379198 @default.
- W2324836477 cites W2018608826 @default.
- W2324836477 cites W2028660819 @default.
- W2324836477 cites W2028821699 @default.
- W2324836477 cites W2040080388 @default.
- W2324836477 cites W2043324182 @default.
- W2324836477 cites W2051057705 @default.
- W2324836477 cites W2053875222 @default.
- W2324836477 cites W2054158258 @default.
- W2324836477 cites W2075873970 @default.
- W2324836477 cites W2079185299 @default.
- W2324836477 cites W2079258402 @default.
- W2324836477 cites W2083676321 @default.
- W2324836477 cites W2091900116 @default.
- W2324836477 cites W2095171944 @default.
- W2324836477 cites W2100232103 @default.
- W2324836477 cites W2142409931 @default.
- W2324836477 cites W2159122978 @default.
- W2324836477 cites W2328540890 @default.
- W2324836477 doi "https://doi.org/10.1021/ac5006487" @default.
- W2324836477 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24758236" @default.
- W2324836477 hasPublicationYear "2014" @default.
- W2324836477 type Work @default.
- W2324836477 sameAs 2324836477 @default.
- W2324836477 citedByCount "48" @default.
- W2324836477 countsByYear W23248364772015 @default.
- W2324836477 countsByYear W23248364772016 @default.
- W2324836477 countsByYear W23248364772017 @default.
- W2324836477 countsByYear W23248364772018 @default.
- W2324836477 countsByYear W23248364772019 @default.
- W2324836477 countsByYear W23248364772020 @default.
- W2324836477 countsByYear W23248364772021 @default.
- W2324836477 countsByYear W23248364772022 @default.
- W2324836477 countsByYear W23248364772023 @default.
- W2324836477 crossrefType "journal-article" @default.
- W2324836477 hasAuthorship W2324836477A5035405996 @default.
- W2324836477 hasAuthorship W2324836477A5073511552 @default.
- W2324836477 hasAuthorship W2324836477A5075509015 @default.
- W2324836477 hasAuthorship W2324836477A5090892663 @default.
- W2324836477 hasConcept C111368507 @default.
- W2324836477 hasConcept C127313418 @default.
- W2324836477 hasConcept C147789679 @default.
- W2324836477 hasConcept C160756335 @default.
- W2324836477 hasConcept C17525397 @default.
- W2324836477 hasConcept C185592680 @default.
- W2324836477 hasConcept C2777197210 @default.
- W2324836477 hasConcept C2777289219 @default.
- W2324836477 hasConcept C2986274086 @default.
- W2324836477 hasConcept C31903555 @default.
- W2324836477 hasConcept C43617362 @default.
- W2324836477 hasConcept C55493867 @default.
- W2324836477 hasConceptScore W2324836477C111368507 @default.
- W2324836477 hasConceptScore W2324836477C127313418 @default.
- W2324836477 hasConceptScore W2324836477C147789679 @default.
- W2324836477 hasConceptScore W2324836477C160756335 @default.
- W2324836477 hasConceptScore W2324836477C17525397 @default.
- W2324836477 hasConceptScore W2324836477C185592680 @default.
- W2324836477 hasConceptScore W2324836477C2777197210 @default.
- W2324836477 hasConceptScore W2324836477C2777289219 @default.
- W2324836477 hasConceptScore W2324836477C2986274086 @default.
- W2324836477 hasConceptScore W2324836477C31903555 @default.
- W2324836477 hasConceptScore W2324836477C43617362 @default.
- W2324836477 hasConceptScore W2324836477C55493867 @default.
- W2324836477 hasIssue "9" @default.
- W2324836477 hasLocation W23248364771 @default.
- W2324836477 hasLocation W23248364772 @default.
- W2324836477 hasOpenAccess W2324836477 @default.
- W2324836477 hasPrimaryLocation W23248364771 @default.
- W2324836477 hasRelatedWork W1990083472 @default.
- W2324836477 hasRelatedWork W1994161880 @default.
- W2324836477 hasRelatedWork W2010974605 @default.
- W2324836477 hasRelatedWork W2031402104 @default.
- W2324836477 hasRelatedWork W2038544393 @default.
- W2324836477 hasRelatedWork W2052245638 @default.
- W2324836477 hasRelatedWork W2074805488 @default.
- W2324836477 hasRelatedWork W2118020841 @default.
- W2324836477 hasRelatedWork W2160447482 @default.
- W2324836477 hasRelatedWork W2338104549 @default.
- W2324836477 hasVolume "86" @default.
- W2324836477 isParatext "false" @default.
- W2324836477 isRetracted "false" @default.
- W2324836477 magId "2324836477" @default.
- W2324836477 workType "article" @default.