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• W2324841046 abstract "Stretching experiments on lambda-deoxyribonucleic acid (DNA) were carried out in microfluidic channel using a microscope, micro-flow pump, and electromagnet. The micro-flow channel was fabricated by using micro electromechanical technology, and the electromagnet was analyzed by software to simulate the magnetic field distribution. The magnetic field was 248.6 gauss at a current of 0.5 A, and the system could exert 20.4 pN of force on a magnetic bead with diameter of 2.8 μm. When the buffer temperature was 25 °C, the average length of lambda-DNA molecules was 1.9 μm without magnetic field. In a magnetic field of 248.6 gauss, the extension was 7.2 μm, the total length was 9.1 μm, and the coefficient of elasticity was 2.83 × 10−6 N/m. When the buffer temperature was 45 °C, the average length was 4.6 μm without magnetic field. At 45 °C in a magnetic field of 248.6 gauss, the extension was 11.9 μm, the total length was 16.5 μm, and the coefficient of elasticity was 1.71 × 10−6 N/m. Besides, the denature temperature of dsDNA decreases with increasing magnetic force, and the temperature difference can be higher than 7 °C. This system can be used to measure the related properties of macromolecules." @default.
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• W2324841046 date "2016-04-01" @default.
• W2324841046 modified "2023-09-24" @default.
• W2324841046 title "Extension and denature temperature of dsDNA molecules at various magnetic forces by using magnetic tweezers" @default.
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• W2324841046 doi "https://doi.org/10.1049/mnl.2015.0388" @default.
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