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- W2324888907 abstract "Vanillin-acid reagent spectophotometric methods is usually used to detect catechins content,but its accuracy has been questioned.Solvent extraction,TLC and HPLC analysis were adopted to investigate the specificity and accuracy of vanillin-acid reagent reaction.The results showed that it would not interfere with the determination of catechins because the sensitivity of catechins was the highest compared to those substances such as flavonols,flavanonols and tea saponin.Some factors influencing the reactions of vanillin-acid reagent with catechins,including the kinds of acid,acid concentration,reaction temperature and reaction time were investigated.The results suggested that the best method to analyze catechins content should be performed by using 1% vanillin-HCl reagent and reacting for 5 min under room temperature.Catechins content could be calculated with the formula of Y(A505nm)=aX+b." @default.
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- W2324888907 date "2010-01-01" @default.
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- W2324888907 title "Determination of catechins in tea plants with vanillin-acid reagent." @default.
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