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- W232508502 abstract "A highly sensitive, solid-phase radioimmunoassay has been developed for detecting platelet-bound immunoglobulin, which employs /sup 125/I-staphylococcal protein A. The assay detects platelet IgG at the picogram level, which is 10- to 50-fold more sensitive than currently available procedures. It is relatively simple and can be performed on frozen extracts from as little as 2 x 10/sup 5/ platelets. Washed platelets are frozen, thawed, sonicated, and centrifuged at 20,000 x g. The supernatant, which contains 7S IgG, is applied in serial dilution to the wells of a plastic microliter plate capable of adsorbing protein. Commercial rabbit anti-human IgG is added and then sandwiched to /sup 125/I-staphylococcal protein A. The wells are removed from the plate and assayed for radioactivity. Thirty-two of 35 thrombocytopenic patients (92%) with ATP had platelet IgG values greater than those of controls by 2 S.D. and averaged 150 +- 145 (S.D.) ng/10/sup 6/ platelets; 11 healthy controls, 11.4 +- 7.4 ng; five thrombocytopenic controls, 9.7 +- 13 ng. The platelet count (X) correlated inversely with IgG/platelet (Y) according to the equation: Log Y = -0.66 log X + 4.8; r = -0.71, p < 0.001. Elevated platelet IgG levels were also found in 5 ATP patients in more » apparent remission; two of four had evidence for compensated thrombocytolysis (increased megathrombocytes). Elevated platelet IgG levels were also found in six patients with thrombocytopenia secondary to lymphoproliferative disorders. « less" @default.
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- W232508502 date "1979-10-01" @default.
- W232508502 modified "2023-09-24" @default.
- W232508502 title "Solid-phase radioimmunoassay for bound anti-platelet antibody. Studies on 45 patients with autoimmune platelet disorders. [/sup 125/I, PBI]" @default.
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