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• W2325188889 endingPage "2647" @default.
• W2325188889 startingPage "2638" @default.
• W2325188889 abstract "The binding of permethrin (PE) with calf thymus DNA (ctDNA) in physiological buffer (pH 7.4) was investigated by ultraviolet–visible (UV–vis) absorption, fluorescence, circular dichroism (CD), and Fourier transform infrared (FT–IR) spectroscopy merging with multivariate curve resolution–alternating least-squares (MCR–ALS) chemometrics approach. The MCR–ALS was applied to resolve the combined spectroscopic data matrix, which was obtained by UV–vis and fluorescence methods. The concentration profiles of PE, ctDNA, and PE–ctDNA complex and their pure spectra were then successfully obtained. The PE molecular was found to be able to intercalate into the base pairs of ctDNA as evidenced by decreases in resonance light-scattering signal and iodide-quenching effect and increase in ctDNA viscosity. The results of FT–IR spectra indicated that PE was prone to bind to G–C base pairs of ctDNA, and the molecular docking studies were used to validate and clarify the specific binding. The observed changes in CD signals revealed that the DNA turned into a more highly wound form of B-conformation. The calculated thermodynamic parameters, enthalpy change (ΔH°) and entropy change (ΔS°), suggested that hydrogen bonds and van der Waals forces played a predominant role in the binding of PE to ctDNA." @default.
• W2325188889 created "2016-06-24" @default.
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• W2325188889 date "2013-03-07" @default.
• W2325188889 modified "2023-09-27" @default.
• W2325188889 title "Probing the Binding of Insecticide Permethrin to Calf Thymus DNA by Spectroscopic Techniques Merging with Chemometrics Method" @default.
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• W2325188889 doi "https://doi.org/10.1021/jf400017f" @default.
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• W2325188889 hasPublicationYear "2013" @default.
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