Matches in SemOpenAlex for { <https://semopenalex.org/work/W2325382300> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2325382300 abstract "Background:Oral aromatase inhibitors (AI) and to a lesser extend tamoxifen can promote recovery of ovarian function in postmenopausal breast cancer patients. Biochemical monitoring of ovarian function is important as folliculogenesis may lead to pregnancy, vaginal bleeding and loss of efficacy of the oral AI. Biochemical monitoring of oestradiol in exemestane users is problematic because exemestane metabolites cross react in most immunoassays for oestradiol. Following our finding of luteal phase progesterone levels in serum of some postmenopausal women with vaginal bleeding on exemestane, we assessed potential cross reactivity between exemestane and its 4-OH metabolite in the Roche Elecsys® progesterone assay. We also studied the frequency of such progesterone activity in serum from consecutive exemestane users and correlated values with FSH.Methods:Progesterone concentrations were measured with the Elecsys® assay in blanco serum, blanco serum with pure exemestane and blanco serum with the pure 17-OH-exemestane metabolite. Several concentrations of the purifided 17-OH-exemestane metabolite in DSMO solvent were then prepared using the Elecsys Diluent MultiAssay and progesterone concentration was measured in these samples using the Elecsys® assay. Cross sectional blood samples from 94 exemestane users were measured for progesterone activity with this assay and also for FSH using the Roche Modular E170® (F. Hoffmann-La Roche Ltd, Basel, Switserland).Results:Using this progesterone assay, 41% of exemestane users had luteal phase progesterone levels (>1.7 ng/mL). There is cross reactivity with exemestane but more importantly with the 17-OH metabolite (table). The level of progesterone and cross reactivity for 2 known concentrations of the 17-OH-metabolite are also shown in this table. There is a correlation between progesterone picked up by the Elecsys® assay and FSH which increased with progesterone activity.Conclusion:Progesterone levels in exemestane users measured with the Elecsys® assay from Roche were often overestimated due to cross reactivity with exemestane and its main metabolite. This progesterone assay can not be used to define menopause or distinguish postmenopausal from menstrual bleeding in exemestane users. Higher FSH levels with higher progesterone activity in this assay suggest a link between circulating exemestane/metabolites and the quantitative downregulation of postmenopausal estrogens by this AI, but serial rather than cross sectional measurements are required to confirm this.We acknowledge Pfizer for providing us with pure exemestane and 17-OH-metabolite. Citation Information: Cancer Res 2009;69(24 Suppl):Abstract nr 5147." @default.
- W2325382300 created "2016-06-24" @default.
- W2325382300 creator A5001188276 @default.
- W2325382300 creator A5007579054 @default.
- W2325382300 creator A5008071748 @default.
- W2325382300 creator A5010127174 @default.
- W2325382300 creator A5020225283 @default.
- W2325382300 creator A5025970751 @default.
- W2325382300 creator A5048282050 @default.
- W2325382300 creator A5066491996 @default.
- W2325382300 creator A5086320066 @default.
- W2325382300 date "2009-12-15" @default.
- W2325382300 modified "2023-09-27" @default.
- W2325382300 title "Cross Reactivity between the Roche Elecsys® Progesterone Assay and Exemestane." @default.
- W2325382300 doi "https://doi.org/10.1158/0008-5472.sabcs-09-5147" @default.
- W2325382300 hasPublicationYear "2009" @default.
- W2325382300 type Work @default.
- W2325382300 sameAs 2325382300 @default.
- W2325382300 citedByCount "0" @default.
- W2325382300 crossrefType "proceedings-article" @default.
- W2325382300 hasAuthorship W2325382300A5001188276 @default.
- W2325382300 hasAuthorship W2325382300A5007579054 @default.
- W2325382300 hasAuthorship W2325382300A5008071748 @default.
- W2325382300 hasAuthorship W2325382300A5010127174 @default.
- W2325382300 hasAuthorship W2325382300A5020225283 @default.
- W2325382300 hasAuthorship W2325382300A5025970751 @default.
- W2325382300 hasAuthorship W2325382300A5048282050 @default.
- W2325382300 hasAuthorship W2325382300A5066491996 @default.
- W2325382300 hasAuthorship W2325382300A5086320066 @default.
- W2325382300 hasConcept C121608353 @default.
- W2325382300 hasConcept C126322002 @default.
- W2325382300 hasConcept C134018914 @default.
- W2325382300 hasConcept C143228043 @default.
- W2325382300 hasConcept C185592680 @default.
- W2325382300 hasConcept C2775860665 @default.
- W2325382300 hasConcept C2776166826 @default.
- W2325382300 hasConcept C2777477808 @default.
- W2325382300 hasConcept C530470458 @default.
- W2325382300 hasConcept C71315377 @default.
- W2325382300 hasConcept C71924100 @default.
- W2325382300 hasConceptScore W2325382300C121608353 @default.
- W2325382300 hasConceptScore W2325382300C126322002 @default.
- W2325382300 hasConceptScore W2325382300C134018914 @default.
- W2325382300 hasConceptScore W2325382300C143228043 @default.
- W2325382300 hasConceptScore W2325382300C185592680 @default.
- W2325382300 hasConceptScore W2325382300C2775860665 @default.
- W2325382300 hasConceptScore W2325382300C2776166826 @default.
- W2325382300 hasConceptScore W2325382300C2777477808 @default.
- W2325382300 hasConceptScore W2325382300C530470458 @default.
- W2325382300 hasConceptScore W2325382300C71315377 @default.
- W2325382300 hasConceptScore W2325382300C71924100 @default.
- W2325382300 hasLocation W23253823001 @default.
- W2325382300 hasOpenAccess W2325382300 @default.
- W2325382300 hasPrimaryLocation W23253823001 @default.
- W2325382300 hasRelatedWork W122974628 @default.
- W2325382300 hasRelatedWork W127087446 @default.
- W2325382300 hasRelatedWork W1975185849 @default.
- W2325382300 hasRelatedWork W2018642629 @default.
- W2325382300 hasRelatedWork W2034860277 @default.
- W2325382300 hasRelatedWork W2047416552 @default.
- W2325382300 hasRelatedWork W2068057248 @default.
- W2325382300 hasRelatedWork W2107114875 @default.
- W2325382300 hasRelatedWork W2139273711 @default.
- W2325382300 hasRelatedWork W2145283112 @default.
- W2325382300 hasRelatedWork W2148569448 @default.
- W2325382300 hasRelatedWork W2149870042 @default.
- W2325382300 hasRelatedWork W2170185740 @default.
- W2325382300 hasRelatedWork W2344642477 @default.
- W2325382300 hasRelatedWork W2466351826 @default.
- W2325382300 hasRelatedWork W2632944447 @default.
- W2325382300 hasRelatedWork W2891168916 @default.
- W2325382300 hasRelatedWork W2991935131 @default.
- W2325382300 hasRelatedWork W3041176795 @default.
- W2325382300 hasRelatedWork W2827030189 @default.
- W2325382300 isParatext "false" @default.
- W2325382300 isRetracted "false" @default.
- W2325382300 magId "2325382300" @default.
- W2325382300 workType "article" @default.