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- W2325572502 abstract "Abstract An artificial site‐selective DNA cutter to hydrolyze single‐stranded DNA at a desired site was prepared from Ce IV /ethylenediamintetraacetic acid (EDTA) and two ethylenediamine‐ N,N,N′,N′ ‐tetrakis(methylenephosphonic acid)–oligonucleotide conjugates. By using this cutter, the sense strand of a blue fluorescent protein (BFP) gene was selectively cut at a predetermined site in the chromophore‐coding region. The upstream fragment obtained by the site‐selective scission was ligated with the downstream fragment of the closely related green fluorescent protein (GFP) gene so that the 5′‐ and 3′‐end portions of the chromophore came from the BFP fragment and the GFP fragment, respectively. The recombinant gene was successfully expressed in E. coli and the chimeric chromophore emitted green fluorescence as expected." @default.
- W2325572502 created "2016-06-24" @default.
- W2325572502 creator A5026544069 @default.
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- W2325572502 date "2011-07-13" @default.
- W2325572502 modified "2023-10-18" @default.
- W2325572502 title "Manipulation of Single-Stranded DNA by Using an Artificial Site-Selective DNA Cutter Composed of Cerium(IV)/EDTA and Phosphonate-Oligonucleotide Conjugates" @default.
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- W2325572502 doi "https://doi.org/10.1002/asia.201100238" @default.
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